Abstract Shellfish allergy is a crucial public health problem and a severe food safety issue, resulting in an urgent need for an accurate, simple and sensitive method to detect the shellfish allergens in complex food systems. Herein, we have desighed an “on-off-on” fluorescence aptasensor based on fluorescence resonance energy transfer (FRET) using prepared carboxyl groups functionalized CQDs (cCQDs) and graphene oxide (GO) for the detection and quantification of the major shellfish allergen arginine kinase (AK). The FRET aptasensor allowed for the sensitive detection of AK in the range of 0.001–10 μg/mL with the limit of detection (LOD) of 0.14 ng/mL (S/N = 3), and the limit of quantitation (LOQ) of 0.27 ng/mL (S/N = 10). This reliable, accurate and specific aptasensor with user-friendly operation may provide new perspectives in the application of fluorescence technologies for food allergens detection in the field of food safety.

中文翻译：

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使用碳量子点和氧化石墨烯的“开-关-开”荧光适体传感器，用于超灵敏检测主要贝类过敏原精氨酸激酶

摘要 贝类过敏是严重的公共卫生问题和严重的食品安全问题，迫切需要一种准确、简单、灵敏的方法来检测复杂食品系统中的贝类过敏原。在此，我们设计了一种基于荧光共振能量转移 (FRET) 的“开-关-开”荧光适体传感器，使用制备的羧基官能化 CQD (cCQD) 和氧化石墨烯 (GO) 来检测和定量主要贝类过敏原精氨酸激酶（AK）。FRET 适体传感器允许在 0.001–10 μg/mL 范围内灵敏检测 AK，检测限 (LOD) 为 0.14 ng/mL (S/N = 3)，定量限 (LOQ) 为0.27 纳克/毫升（信噪比 = 10）。这个靠谱，