Abstract Reliable trace exosomal miRNA detection remains challenging. Herein, we established a universal Target Initiate Proximity Ligation Assay (TIPLA), to sensitively detect miRNA by integrating target miRNA initiate cycle and proximity ligation triggered rolling circle amplification (RCA). The outstanding features of this approach is calculated as: i) three signal amplification composed by target miRNA cycle, proximity ligation initiated RCA and magnet-based enrichment ensure high-sensitive detection of exosomal miRNA with a sensitivity of 30.25 fM; ii) direct detection of miRNAs without additional reverse transcription procedure inevitable in conventional nucleic acid detection methods. This dual amplification made TIPLA a sensitive approach to detect exosomal miRNA from both culture cells and cardiovascular disease patients, revealing a potential universal molecular detection platform for the screening, diagnosis, and prognosis prediction of multiple diseases.

中文翻译：

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使用靶启动邻近连接试验 (TIPLA) 灵敏检测心血管疾病的外泌体 miRNA

摘要 可靠的微量外泌体 miRNA 检测仍然具有挑战性。在此，我们建立了一个通用的靶启动邻近连接分析（TIPLA），通过整合目标 miRNA 启动循环和邻近连接触发滚环扩增（RCA）来灵敏地检测 miRNA。该方法的突出特点计算为：i) 由目标 miRNA 循环、邻近连接启动的 RCA 和基于磁体的富集组成的三个信号放大确保外泌体 miRNA 的高灵敏度检测，灵敏度为 30.25 fM；ii) 无需额外的逆转录程序即可直接检测 miRNA，这在常规核酸检测方法中是不可避免的。这种双重扩增使 TIPLA 成为检测培养细胞和心血管疾病患者外泌体 miRNA 的灵敏方法，