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Nitrogen Catabolite Repression in members of Paracoccidioides complex.
Microbial Pathogenesis ( IF 3.3 ) Pub Date : 2020-06-23 , DOI: 10.1016/j.micpath.2020.104281
Vanessa Rafaela Milhomem Cruz-Leite 1 , Silvia Maria Salem-Izacc 1 , Evandro Novaes 2 , Bruno Junior Neves 3 , Wesley de Almeida Brito 4 , Lana O'Hara Souza Silva 1 , Juliano Domiraci Paccez 1 , Juliana Alves Parente-Rocha 1 , Maristela Pereira 1 , Célia Maria de Almeida Soares 1 , Clayton Luiz Borges 1
Affiliation  

Paracoccidioides complex is a genus that comprises pathogenic fungi which are responsible by systemic disease Paracoccidioidomycosis. In host tissues, pathogenic fungi need to acquire nutrients in order to survive, making the uptake of nitrogen essential for their establishment and dissemination. Nitrogen utilization is employed by the alleviation of Nitrogen Catabolite Repression (NCR) which ensures the use of non-preferential or alternative nitrogen sources when preferential sources are not available. NCR is controlled by GATA transcription factors which act through GATA binding sites on promoter regions in NCR-sensitive genes. This process is responsible for encoding proteins involved with the scavenge, uptake and catabolism of a wide variety of non-preferential nitrogen sources. In this work, we predict the existence of AreA GATA transcription factor and feature the zinc finger domain by three-dimensional structure in Paracoccidioides. Furthermore, we demonstrate the putative genes involved with NCR response by means of in silico analysis. The gene expression profile under NCR conditions was evaluated. Demonstrating that P. lutzii supported transcriptional regulation and alleviated NCR in non-preferential nitrogen-dependent medium. The elucidation of NCR in members of Paracoccidioides complex will provide new knowledge about survival, dissemination and virulence for these pathogens with regard to nitrogen-scavenging strategies in the interactions of host-pathogens.



中文翻译:

副球菌复合体中的氮分解代谢抑制。

副球菌复合体是包含致病性真菌的属,其由系统性疾病副球孢子菌病引起。在宿主组织中,致病真菌需要获取营养才能生存,这使得氮的吸收对于其建立和传播至关重要。氮的利用是通过减轻氮分解代谢抑制作用(NCR)来进行的,这可确保在无法获得优先来源时确保使用非优惠或替代性氮源。NCR由GATA转录因子控制,该转录因子通过NCR敏感基因启动子区域的GATA结合位点起作用。该过程负责编码与多种非优先氮源的清除,吸收和分解代谢有关的蛋白质。在这项工作中副球菌。此外,我们通过计算机分析证明了与NCR反应有关的推定基因。评价了在NCR条件下的基因表达谱。证明P. lutzii支持转录调节并减轻了非优先氮依赖性培养基中的NCR。拟南芥复合体成员中NCR的阐明将提供有关这些病原体在宿主-病原体相互作用中的氮清除策略的存活,传播和毒力的新知识。

更新日期:2020-07-23
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