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Optimized sensitive and inexpensive method to measure D-lactate as a surrogate marker of methylglyoxal fluxes in metabolically relevant contexts
Methods ( IF 4.2 ) Pub Date : 2020-06-01 , DOI: 10.1016/j.ymeth.2020.06.010 Alejandro Gugliucci 1 , Russell Caccavello 1
Methods ( IF 4.2 ) Pub Date : 2020-06-01 , DOI: 10.1016/j.ymeth.2020.06.010 Alejandro Gugliucci 1 , Russell Caccavello 1
Affiliation
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INTRODUCTION
Plasma D-lactate levels can be considered a surrogate indicator of MG flux. There are commercial methods specifically designed to cover pathological ranges (mmol/l in sepsis o leaky gut) that are not suitable to cover the metabolically relevant ranges MATERIAL AND METHODS: We modified and optimized the D-lactate Colorimetric Assay kit MAK058 from Sigma adding an ultracentrifugation step, kinetic reading and increasing reaction temperature RESULTS AND CONCLUSIONS: We present a modified, optimized and validated method to measure serum D-lactate using a commercial kit to increase the sensitivity of the method to 2 orders of magnitude lower as well as minimizing interferences so that it may be used by researchers to explore the D-lactate pathway in metabolic disorders at a low cost (colorimetric method and a plate reader) and with a much higher specificity.
中文翻译：
优化敏感且廉价的方法来测量 D-乳酸作为代谢相关环境中甲基乙二醛通量的替代标记
简介 血浆 D-乳酸水平可被视为 MG 通量的替代指标。有专门设计用于覆盖不适合覆盖代谢相关范围的病理范围（脓毒症或肠漏中的 mmol/l）的商业方法 材料和方法：我们修改和优化了 Sigma 的 D-乳酸比色测定试剂盒 MAK058，添加了一个超速离心步骤、动力学读数和增加反应温度 结果和结论：我们提出了一个改进的，
更新日期：2020-06-01
中文翻译：
优化敏感且廉价的方法来测量 D-乳酸作为代谢相关环境中甲基乙二醛通量的替代标记
简介 血浆 D-乳酸水平可被视为 MG 通量的替代指标。有专门设计用于覆盖不适合覆盖代谢相关范围的病理范围（脓毒症或肠漏中的 mmol/l）的商业方法 材料和方法：我们修改和优化了 Sigma 的 D-乳酸比色测定试剂盒 MAK058，添加了一个超速离心步骤、动力学读数和增加反应温度 结果和结论：我们提出了一个改进的，
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