As an important class of biomaterials，bionics inspired materials has been widely used in creating extracorporeal and implantable medical devices. However, specific service environment is often faced with multiple requirements rather than single function. Herein, we designed a phospholipid-based multifunctional coating with phospholipids-based polymers, type I collagen (Col-I) and Arg-Glu-Asp-Val (REDV) peptide, via layer-by-layer assembly. The successful synthesis of the polymers and the coating is proved by a series of characterization methods including Fourier transforming infrared spectra (FTIR), proton nuclear magnetic resonance (¹H NMR), ultraviolet-visible spectra (UV) and X-ray photoelectron spectroscopy (XPS), while the assembly process and quality change of the coating were monitored via quartz crystal microbalance (QCM). Besides, hydrophilicity and roughness of this coating was analyzed via water contact angle (WCA) and atomic force microscope (AFM), respectively. Finally, results from platelet adhesion, activation assay, smooth muscle cells (SMCs) and endothelial cells (ECs) cultures indicated that the multifunctional coating could strongly inhibit platelet adhesion and SMCs proliferation, hence provide practical application of the coating with good biocompatibility, especially the anticoagulant property and cell compatibility. It is expected that this coating may be used in blood-contacting fields such as cardiovascular stent or other devices in the future.

作为一类重要的生物材料，仿生学启发的材料已广泛用于创建体外和可植入的医疗设备。但是，特定的服务环境通常面临多个需求，而不是单一功能。在本文中，我们通过逐层组装设计了基于磷脂的多功能涂层，其中包含基于磷脂的聚合物，I型胶原蛋白（Col-1）和Arg-Glu-Asp-Val（REDV）肽。通过一系列表征方法（包括傅立叶变换红外光谱（FTIR），质子核磁共振（¹H NMR），紫外可见光谱（UV）和X射线光电子能谱（XPS），同时通过石英晶体微量天平（QCM）监测涂层的组装过程和质量变化。此外，分别通过水接触角（WCA）和原子力显微镜（AFM）分析了该涂层的亲水性和粗糙度。最后，血小板粘附，激活测定，平滑肌细胞（SMCs）和内皮细胞（ECs）培养的结果表明，该多功能涂层可以强烈抑制血小板粘附和SMCs的增殖，因此具有良好的生物相容性，特别是在生物相容性方面提供了实用的涂层应用。抗凝特性和细胞相容性。预期该涂层将来可用于血液接触领域，例如心血管支架或其他装置。