Abstract This study depicts easy process of propolis by co-electrospinning without using any toxic agent for biomedical applications. To achieve this, polyvinyl alcohol was utilized as co-spinning agent to fabricate biomimetic Propolis/PVA scaffold. Here, whilst PVA was used as a supportive material to accumulate propolis in scaffold, propolis was employed to enrich biologic aspect of scaffold. This strategy overcomes challenges of propolis processing originated from solubility problems and offers easy processability of propolis in order to use in biomedical applications. Electrospun Propolis/PVA scaffolds were crosslinked with glutaraldehyde and drop-cast model was utilized as a control. Formation of porous, bead-free nanofiber architectures was confirmed through surface morphology analysis, while drop-cast model shows non-porous morphology. Wettability results confirmed both crosslinking and integration of propolis into polyvinyl alcohol scaffold moved contact angle to hydrophobic region. Presence and amount of propolis in hybrid scaffolds were validated via absorbance spectrum results. Bioactivity and biocompatibility of propolis-enriched scaffolds were analyzed through protein adsorption capacity. Obtained findings are evidence that electrospinning methodology offers easy and biosafe process of propolis. Electrospun Propolis/PVA exhibits desired properties and could be potentially utilized as scaffold for tissue engineering or as a wound dressing graft in biomedical field.

中文翻译：

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一种通过共静电纺丝制造富含蜂胶的仿生 PVA 结构的简便方法

摘要 本研究描述了在不使用任何毒剂的情况下，通过共静电纺丝法制备蜂胶的简单工艺，用于生物医学应用。为了实现这一点，聚乙烯醇被用作共纺剂来制造仿生蜂胶/PVA 支架。在这里，虽然 PVA 被用作在支架中积累蜂胶的支持材料，但蜂胶被用于丰富支架的生物学方面。该策略克服了源于溶解性问题的蜂胶加工挑战，并提供了蜂胶的易于加工性，以便用于生物医学应用。电纺蜂胶/聚乙烯醇支架与戊二醛交联，滴铸模型用作对照。通过表面形态分析证实了多孔、无珠纳米纤维结构的形成，而滴铸模型显示无孔形态。润湿性结果证实了蜂胶与聚乙烯醇支架的交联和整合将接触角移动到疏水区域。通过吸收光谱结果验证了混合支架中蜂胶的存在和数量。通过蛋白质吸附能力分析富含蜂胶的支架的生物活性和生物相容性。获得的发现证明静电纺丝方法提供了简单且生物安全的蜂胶工艺。电纺蜂胶/PVA 表现出所需的特性，有可能用作组织工程的支架或生物医学领域的伤口敷料移植物。通过吸收光谱结果验证了混合支架中蜂胶的存在和数量。通过蛋白质吸附能力分析富含蜂胶的支架的生物活性和生物相容性。获得的发现证明静电纺丝方法提供了简单且生物安全的蜂胶工艺。电纺蜂胶/PVA 表现出所需的特性，有可能用作组织工程的支架或生物医学领域的伤口敷料移植物。通过吸收光谱结果验证了混合支架中蜂胶的存在和数量。通过蛋白质吸附能力分析富含蜂胶的支架的生物活性和生物相容性。获得的发现证明静电纺丝方法提供了简单且生物安全的蜂胶工艺。电纺蜂胶/PVA 表现出所需的特性，有可能用作组织工程的支架或生物医学领域的伤口敷料移植物。