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A novel constructed carbonate-mineralized functional bacterial consortium for high-efficiency cadmium biomineralization.
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2020-06-23 , DOI: 10.1016/j.jhazmat.2020.123269
Tingting Yin 1 , Hai Lin 1 , Yingbo Dong 1 , Bing Li 1 , Yinhai He 1 , Chenjing Liu 1 , Xi Chen 1
Affiliation  

A stable, urease-producing consortium (UPC) was constructed for high-efficiency cadmium (Cd) ion mineralization via a short-term and efficient acclimation process (five acclimation transfers). 16S rRNA gene high-throughput sequencing and quantitative polymerase chain reaction (qPCR) analyses of the urease subunit C (ureC) gene suggested that the three functional genera, all belonging to the phylum Firmicutes, rapidly increased during the process and finally composed the UPC (70.22–75.41 % of Sporosarcina, 13.83–20.66 % of norank_f_Bacillaceae, and 5.91–13.69 % of unclassified_f_Bacillaceae). The UPC exhibited good adaptability to a wide range of environmental conditions (a pH range of 4.0–11.0, temperature range of 10−45 °C, and Cd concentration range of 0−200 mg L−1). After 8 h of incubation, 92.87 % of Cd at an initial concentration of 100 mg L−1 was mineralized by UPC, exhibiting a great improvement as compared to the first acclimated consortium (C-1). Furthermore, although the acclimated consortium had been successively transferred 21 times, the Cd biomineralization efficiency remained stable, and this was consistent with the observed stable microbial community structure. X-ray diffraction (XRD) spectra revealed that Cd was mineralized in a (Ca0.67, Cd0.33)CO3 phase. This research obtained a promising microbial resource for the biomineralization of Cd or other hazardous heavy metal contaminants.



中文翻译:

一种用于高效镉生物矿化的新型构造的碳酸盐矿化功能性细菌财团。

构建了一个稳定的产生脲酶的财团(UPC),用于通过短期和有效的适应过程(五次适应转移)来实现高效镉(Cd)离子矿化。对脲酶亚基C(ureC)基因进行16S rRNA基因高通量测序和定量聚合酶链反应(qPCR)分析表明,这三个功能属均属于Firmicutes门,在此过程中迅速增加并最终组成了UPC(的70.22-75.41%Sporosarcina,norank_f_的13.83-20.66%芽孢杆菌,和unclassified_f_的5.91-13.69%芽孢杆菌)。UPC对各种环境条件(pH范围为4.0-11.0,温度范围为10-45°C,Cd浓度范围为0-200 mg L -1)表现出良好的适应性。温育8小时后,UPC矿化了初始浓度为100 mg L -1的Cd的92.87%,与第一个适应的财团(C-1)相比有很大的改善。此外,尽管适应的财团已被连续转移了21次,但Cd的生物矿化效率仍保持稳定,这与观察到的稳定的微生物群落结构一致。X射线衍射(XRD)光谱显示Cd在(Ca0.67,Cd0.33)CO 3中矿化相。这项研究为Cd或其他有害重金属污染物的生物矿化获得了有希望的微生物资源。

更新日期:2020-07-03
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