A stable, urease-producing consortium (UPC) was constructed for high-efficiency cadmium (Cd) ion mineralization via a short-term and efficient acclimation process (five acclimation transfers). 16S rRNA gene high-throughput sequencing and quantitative polymerase chain reaction (qPCR) analyses of the urease subunit C (ureC) gene suggested that the three functional genera, all belonging to the phylum Firmicutes, rapidly increased during the process and finally composed the UPC (70.22–75.41 % of Sporosarcina, 13.83–20.66 % of norank_f_Bacillaceae, and 5.91–13.69 % of unclassified_f_Bacillaceae). The UPC exhibited good adaptability to a wide range of environmental conditions (a pH range of 4.0–11.0, temperature range of 10−45 °C, and Cd concentration range of 0−200 mg L⁻¹). After 8 h of incubation, 92.87 % of Cd at an initial concentration of 100 mg L⁻¹ was mineralized by UPC, exhibiting a great improvement as compared to the first acclimated consortium (C-1). Furthermore, although the acclimated consortium had been successively transferred 21 times, the Cd biomineralization efficiency remained stable, and this was consistent with the observed stable microbial community structure. X-ray diffraction (XRD) spectra revealed that Cd was mineralized in a (Ca0.67, Cd0.33)CO₃ phase. This research obtained a promising microbial resource for the biomineralization of Cd or other hazardous heavy metal contaminants.

构建了一个稳定的产生脲酶的财团（UPC），用于通过短期和有效的适应过程（五次适应转移）来实现高效镉（Cd）离子矿化。对脲酶亚基C（ureC）基因进行16S rRNA基因高通量测序和定量聚合酶链反应（qPCR）分析表明，这三个功能属均属于Firmicutes门，在此过程中迅速增加并最终组成了UPC（的70.22-75.41％Sporosarcina，norank_f_的13.83-20.66％芽孢杆菌，和unclassified_f_的5.91-13.69％芽孢杆菌）。UPC对各种环境条件（pH范围为4.0-11.0，温度范围为10-45°C，Cd浓度范围为0-200 mg L ^-1）表现出良好的适应性。温育8小时后，UPC矿化了初始浓度为100 mg L ^-1的Cd的92.87％，与第一个适应的财团（C-1）相比有很大的改善。此外，尽管适应的财团已被连续转移了21次，但Cd的生物矿化效率仍保持稳定，这与观察到的稳定的微生物群落结构一致。X射线衍射（XRD）光谱显示Cd在（Ca0.67，Cd0.33）CO _3中矿化相。这项研究为Cd或其他有害重金属污染物的生物矿化获得了有希望的微生物资源。