Marine bacterium Vibrio natriegensis a novel host platform for different applications in molecular biology and biotechnology. It has one of the fastest growth rates of any known microorganisms and its extremely short doubling time indicates a high level of proteosynthetic activity. Regarding the necessity of developing new high-level protein expression systems it represents an extremely interesting subject. V. natriegens fulfills many important features for a suitable host including non- pathogenicity, easy scale-up process, potential for using alternative carbon sources (compared to E. coli), growth media and potential for further genetic and metabolic engineering with employment of a wide range of genetic tools.

This work compares V. natriegens as an expression host for production of recombinant human growth hormone (hGH), yeast alcohol dehydrogenase (ADH) and archaeal catalase-peroxidase (AfKatG) to E. coliand establishes the basis for future development of this platform. The selected proteins are of different origins, sizes and intended applications.

Our results have shown that cultures of V. natriegens using sucrose as a main carbon source can be used for the production of industrially applicable proteins, where it offers higher biomass productions compared to E. coli. In case of human growth hormone production, produced amounts were lower compared to those of E. coli (38 % of total cell protein (TCP) for V. natriegens vs. 58 % of TCP for E. coli, with similar solubility of around 40 % in both cases). In case of yeast alcohol dehydrogenase, V. natriegens produced 26 % of TCP vs. 42 % of TCP in E. coli, but with severely decreased solubility in case of V. natriegens cultures. Finally

V. natriegens cultures were able to produce catalase-peroxidase AfKatG at the level of 33 % of TCP compared to 26 % of TCP in E. coli. Obtained results suggest that there are still significant differences in reliability and ease of use between E. coli and V. natriegens, with latter being more susceptible to condition changes and producing inconsistent results.

海洋细菌弧菌是分子生物学和生物技术中不同应用的新型宿主平台。它是所有已知微生物中生长速度最快的之一，其极短的加倍时间表明其蛋白合成活性很高。关于开发新的高级蛋白质表达系统的必要性，它代表了一个非常有趣的主题。V. natriegens对合适的宿主具有许多重要功能，包括非致病性，易于扩大规模的工艺，使用替代碳源的潜力（与E. coli相比），生长培养基以及通过使用碳纳米管进行进一步遗传和代谢工程的潜力广泛的遗传工具。

这项工作比较V. natriegens作为生产重组人生长激素（hGH），酵母乙醇脱氢酶（ADH）和古细菌过氧化氢酶-过氧化物酶（AfKatG）至表达宿主大肠杆菌和建立用于该平台的未来发展的基础。所选择的蛋白质具有不同的来源，大小和预期的应用。

我们的结果表明，使用蔗糖作为主要碳源的纳豆弧菌培养物可用于生产工业适用的蛋白质，与大肠杆菌相比，该蛋白质可提供更高的生物量生产。在产生人类生长激素的情况下，产生的量要比大肠杆菌要低（纳豆弧菌总细胞蛋白（TCP）的38％与大肠杆菌的TCP的58％相比，溶解度约为40在两种情况下均为％）。在酵母酒精脱氢酶的情况下，纳氏弧菌产生了26％的TCP，而在大肠杆菌中产生了42％的TCP ，但是在纳氏弧菌的情况下溶解度大大降低文化。最后

V. natriegens培养物能够产生过氧化氢酶-过氧化物酶AfKatG，其水平为TCP的33％，而大肠杆菌中只有26％的TCP 。所获得的结果表明，大肠杆菌和纳曲菌之间在可靠性和易用性方面仍存在显着差异，后者更容易受到条件变化的影响并产生不一致的结果。