Phylogeography combines ancestry with location and can be translated to intratumor heterogeneity (ITH) to visualize how tumors spread. ITH is common in human tumors, with many genetic and phenotypic differences between regions. The roles of ITH in progression are uncertain because many subclones lack discernable driver mutations. ITH can be visualized by mapping mutations onto microscopic sections, where subclones are directly associated with phenotypes, especially the deeper areas with the more invasive cells that confer worst clinical outcomes. Instead of a stepwise hierarchy where subclones segregate by phenotype with later branching subclones in more invasive areas, multiple subclones share superficial and invasive phenotype and are jigsaw arrayed in vertical columns. Phylogeography shows that both early and late subclones extend from the surface to the invasive front, suggesting that founder cells start with phenotypic plasticity and essentially all the drivers necessary to rapidly grow into large invasive tumors.

语音记录结合了祖先和位置，可以转化为肿瘤内异质性（ITH）以可视化肿瘤的扩散方式。ITH在人类肿瘤中很常见，在区域之间存在许多遗传和表型差异。ITH在进展中的作用尚不确定，因为许多亚克隆缺乏可识别的驱动程序突变。可以通过将突变定位到微观切片上来观察ITH，其中亚克隆与表型直接相关，尤其是在较深的区域中，具有更强侵袭性的细胞会带来最差的临床结果。多个亚克隆具有表位和侵入表型，并以竖向排列的曲线排列，而不是逐步的层次结构，其中亚克隆按表型分离，随后的分支亚克隆在更具侵入性的区域中。