This study investigates the effects of cryopreservation and supplementation of buffalo's semen with Caffeic acid. It studies the effects of different Caffeic acid concentrations on cryopreservation capacity of the buffalo and evaluates their influence on various sperm parameters like motility, viability, progressive motility, sperm plasma membrane integrity, and antioxidant status. Twenty-four semen samples were collected with an artificial vaginal from three adult water buffalos. The semen samples were evaluated and the qualified ejaculates were separated and were diluted in a Tris-based extender. The resulting samples were classified into 5 groups: No antioxidant (control), Control sham (NaOH), Caffeic acid 50 μM, Caffeic acid 100 μM, and Caffeic acid 200 μM. The semen samples encountered cryodamage and the quality was deteriorating during the cryopreservation (P < 0.05). The semen evaluation after thawing showed that the groups of samples receiving 100 μM Caffeic acid had higher viability, total motility, and lower abnormal sperm and better linearity (LIN), curvilinear velocity (VCL), straight-line velocity (VSL) and path velocity and higher intact plasma membrane (P < 0.05) compared to other groups. It is notable that adding 100 μM Caffeic acid to freezing extenders enhances the CAT, GPx, SOD, and GSH and also ameliorates total antioxidant capacity of spermatozoa after thawing. It is notable that the addition of 100 μM Caffeic acid decreases the amount of Malondialdehyde. These reactions lead us to conclude that 100 μM Caffeic acid enhances the semen quality of water buffalo after thawing.

中文翻译：

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咖啡酸可改善冻融过程后水牛 (Bubalus bubalis) 公牛精液的微观精子参数和抗氧化状态

本研究调查冷冻保存和用咖啡酸补充水牛精液的效果。它研究了不同咖啡酸浓度对水牛冷冻保存能力的影响，并评估了它们对各种精子参数的影响，如运动性、活力、渐进性运动、精子质膜完整性和抗氧化状态。用人工阴道从三头成年水牛中收集了 24 份精液样本。评估精液样本，分离合格的精液并在基于 Tris 的稀释剂中稀释。所得样品分为 5 组：无抗氧化剂（对照）、对照组（NaOH）、咖啡酸 50 μM、咖啡酸 100 μM 和咖啡酸 200 μM。精液样本在冷冻保存过程中遇到冷冻损伤，质量下降（P < 0.05）。解冻后的精液评估表明，接受 100 μM 咖啡酸的样品组具有更高的活力、总运动力和更低的异常精子，以及更好的线性 (LIN)、曲线速度 (VCL)、直线速度 (VSL) 和路径速度与其他组相比，完整的质膜 (P < 0.05) 更高。值得注意的是，向冷冻补充剂中添加 100 μM 咖啡酸可增强 CAT、GPx、SOD 和 GSH，并改善解冻后精子的总抗氧化能力。值得注意的是，添加 100 μM 咖啡酸会降低丙二醛的量。这些反应使我们得出结论，100 μM 咖啡酸可提高水牛解冻后的精液质量。