Recently reported biomaterial-based approaches toward prevascularizing tissue constructs rely on biologically or structurally complex scaffolds that are complicated to manufacture and sterilize, and challenging to customize for clinical applications. In the current work, a prevascularization method for soft tissue engineering that uses a non-patterned and non-biological scaffold is proposed. Human fibroblasts and HUVECs were seeded on an ionomeric polyurethane-based hydrogel and cultured for 14 days under medium perfusion. A flow rate of 0.05 mL/min resulted in a greater lumen density in the constructs relative to 0.005 and 0.5 mL/min, indicating the critical importance of flow magnitude in establishing microvessels. Constructs generated at 0.05 mL/min perfusion flow were implanted in a mouse subcutaneous model and intravital imaging was used to characterize host blood perfusion through the construct after 2 weeks. Engineered microvessels were functional (i.e. perfused with host blood and non-leaky) and neovascularization of the construct by host vessels was enhanced relative to non-prevascularized constructs. We report on the first strategy toward engineering functional microvessels in a tissue construct using non-bioactive, non-patterned synthetic polyurethane materials.

最近报道的基于生物材料的使组织构建物预血管化的方法依赖于生物学或结构复杂的支架，这些支架的制造和灭菌很复杂，并且难以定制用于临床应用。在当前的工作中，提出了一种使用非模式和非生物支架的用于软组织工程的血管形成前方法。将人成纤维细胞和HUVEC接种在基于离聚物的聚氨酯水凝胶上，并在中等灌注下培养14天。相对于0.005和0.5 mL / min，0.05 mL / min的流速导致结构中的管腔密度更高，这表明在建立微血管中流速的至关重要。在0生成的构造。将05mL / min的灌注流植入小鼠皮下模型中，并在2周后使用活体成像来表征通过构建体的宿主血液灌注。改造的微血管是功能性的（即，灌注了宿主血液并且没有渗漏），相对于未血管化前的构建体，宿主血管对构建体的新血管形成得到了增强。我们报告了使用非生物活性，无图案的合成聚氨酯材料对组织构造中的功能性微血管进行工程改造的第一种策略。