Abstract Species-dependent mechanisms of morphological development affect filamentous fungi cultivations. In order to influence fungal morphology microparticle-enhanced cultivations (MPEC) were used towards four species exhibiting various mechanisms of morphology formation: a spore agglomerative Aspergillus terreus, hyphae agglomerative Penicillium rubens, spore and hyphae agglomerative Chaetomium globosum and non-agglomerative Mucor racemosus. The experiments were performed in stirred tank bioreactors as one-stage (inoculation with spores) and two-stage (inoculation with 24 -h shake flask preculture) MPEC and standard processes. Fungal morphology was quantified with the use of image analysis. The addition of Al2O3 microparticles to the bioreactor cultures changed the size and shape of mycelial objects of spore agglomerating A. terreus and non-agglomerating M. racemosus the most. The simultaneous action of microparticles and mechanical forces was the strongest for C. globosum, the fungus that combines both hyphae and spore agglomeration mechanisms of morphological development. The weakest effect of microparticles was observed for P. rubens, whose mechanism is hyphae agglomerative. Ultimately, MPEC was more effective in two-stage processes.

中文翻译：

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搅拌罐生物反应器中真菌微粒增强培养的性能取决于物种和工艺阶段的数量

摘要 形态发育的物种依赖性机制影响丝状真菌的培养。为了影响真菌形态，微粒增强培养 (MPEC) 被用于四种表现出不同形态形成机制的物种：孢子团聚土曲霉、菌丝团聚红青霉、孢子和菌丝团聚毛球壳菌和非团聚毛霉菌。实验在搅拌罐生物反应器中作为一级（接种孢子）和两级（接种 24 小时摇瓶预培养）MPEC 和标准过程进行。使用图像分析对真菌形态进行量化。向生物反应器培养物中添加 Al2O3 微粒改变了孢子团聚 A 菌丝体的大小和形状。terreus 和非附聚的 M. racemosus 最多。微粒和机械力的同时作用对于 C. globosum 是最强的，这种真菌结合了形态发育的菌丝和孢子团聚机制。观察到微粒对 P. rubens 的影响最弱，其机制是菌丝团聚。最终，MPEC 在两阶段过程中更有效。