Aurantiochytrium sp. has been developed for commercial docosahexaenoic (DHA) production but the production cost remains a major issue due to exorbitant cultivation substrates. In this study, we explore the capacity of locally isolated Aurantiochytrium sp. to produce DHA utilizing low-cost, safe and extensively abundant fruit extracts derived from rejected fruits as alternative carbon sources via development of several biotechnological processes including; (1) simple and cost-effective sugar extraction from the fruit extracts, (2) screening of the potential fruit extracts and (3) process optimization using selected fruit extract to enhance the DHA yield and (4) comparative study of the selected fruit extract with commercial glucose and fructose to evaluate its competency for large scale production. The capability of Aurantiochytrium sp. SW1 to utilize the fruit extracts were screened and results showed that Aurantiochytrium sp. SW1 produced 20–60% higher lipid and DHA when grown in autoclaved assisted Ananas comosus MD2 extracts in comparison to other fruit extracts. In addition, lipid and DHA production cultivated with Ananas comosus MD2 extract was also 15–50% higher than that of commercial glucose and fructose. This superior production of lipid and DHA of Aurantiochytrium sp. SW1 cultivated in Ananas comosus MD2 extract was shown to probably be caused by significant elevation of the key metabolic enzymes involved in lipid biosynthesis, including malic enzyme, glucose-6-phosphate and ATP citrate lyase and the lowering of the reactive oxygen species and lipid peroxidation activities. Efficient DHA biosynthetic capacity of 0.31 (g DHA/g Biomass) was achieved in this study, suggesting Ananas comosus MD2 extract as an ideal alternative carbon source for economic production of DHA by Aurantiochytrium sp.

金棘藻 已经开发了用于商业二十二碳六烯（DHA）生产的产品，但是由于过多的栽培基质，生产成本仍然是主要问题。在这项研究中，我们探索了局部分离的金眼鱼的能力sp。通过开发多种生物技术工艺，利用廉价，安全且丰富的水果废品中提取的DHA作为替代碳源来生产DHA；（1）从水果提取物中进行简单且经济高效的糖提取，（2）筛选潜在的水果提取物，（3）使用所选水果提取物提高DHA产量的工艺优化，以及（4）所选水果提取物的比较研究与商业葡萄糖和果糖一起评估其大规模生产的能力。Aurantiochytrium sp。的能力。筛选了利用果实提取物的SW1菌株，结果表明该菌株为金葡萄菌。在高压灭菌的辅助凤梨中生长时，SW1产生的脂质和DHA增加20–60％与其他水果提取物相比，MD2提取物。此外，用Ananas comosus MD2提取物培养的脂质和DHA产量也比商品葡萄糖和果糖高15–50％。这种卓越的脂质和金鱼眼DHA的生产。结果表明，在Anaanas comosus MD2提取物中培养的SW1可能是由于参与脂质生物合成的关键代谢酶（包括苹果酸酶，6-磷酸葡萄糖和ATP柠檬酸裂解酶）的显着升高以及活性氧和脂质过氧化作用的降低引起的活动。在这项研究中达到了0.31（g DHA / g生物质）的有效DHA生物合成能力，这暗示了Anaanas comosusMD2提取物是Aurantiochytrium sp。生产DHA的理想替代碳源。