This paper systematically studied the extraction process of total flavonoids from quinoa and the kinetics of macroporous resin purification. On the basis of single-factor experiments, the optimum extraction and purification process of total flavonoids from quinoa was studied by orthogonal experiments. The results showed that the optimum extraction conditions were as follows: ultrasonic time 25 min, temperature 200 °F, solid-liquid ratio 1:10 (g/mL), reflux time 2 h, and ethanol concentration 90%. Under this condition, the total flavonoids extracted from quinoa was 3.859 mg/g. Fifteen percent NaCl was added to salt out protein from flavonoid solution. The best macroporous resin purification process was as follows: pH 2, flow rate 2 BV/h, 0.3 mg/mL flavonoid solution loading, 8 BV water wash with acetone elution. Under this condition, the adsorption rate of purified flavonoids was 80.89%. The purity can be increased from 7.09% of the initial extract to 28.48%.

本文系统地研究了藜麦中总黄酮的提取工艺及大孔树脂纯化的动力学。在单因素实验的基础上，通过正交试验研究了藜麦中总黄酮的最佳提取纯化工艺。结果表明，最佳提取条件为：超声时间25 min，温度200°F，固液比1:10（g / mL），回流时间2 h，乙醇浓度90％。在这种条件下，从藜麦中提取的总黄酮含量为3.859 mg / g。加入15％的氯化钠以从类黄酮溶液中盐析蛋白质。最佳的大孔树脂纯化工艺如下：pH 2，流速2 BV / h，类黄酮溶液上样量0.3 mg / mL，用丙酮洗脱的8 BV水洗。在这种情况下 纯化的类黄酮的吸附率为80.89％。纯度可以从初始提取物的7.09％提高到28.48％。