A number of kinesin proteins (KIFs) have been implicated in the development of multiple cancers. However, little is known about the expression and function of KIF15 in human breast cancer. Herein, we detected KIF15 expression in breast cancer tissues and paired adjacent normal tissues using immunohistochemistry and quantitative real-time polymerase chain reaction analysis, and the correlation of KIF15 expression with clinicopathological parameters was evaluated statistically. The role of KIF15 in cell proliferation, migration, tumor growth and metastasis of breast cancer cells was investigated in vitro and in vivo, and we explored potential molecular mechanisms underlying the effects of KIF15 in breast cancer through western blot analysis. The results revealed that increased KIF15 expression in breast cancer tissues were positively related with tumor size, lymph node metastasis and TNM stage, and higher KIF15 expression predicts a worse prognosis of patients with breast cancer. Furthermore, KIF15 knockdown markedly attenuated breast cancer cell proliferation, migration, tumor growth and metastasis in vitro and in vivo, and silenced KIF15 expression significantly inhibited the expression of phosphorylated AKT, phosphorylated JNK, and cyclin D1, while both p53 and p21 protein expressions were strongly enhanced. These results suggest that KIF15 is a potential oncogene in human breast cancer.

许多驱动蛋白（KIFs）已与多种癌症的发展有关。然而，关于KIF15在人乳腺癌中的表达和功能的了解甚少。在本文中，我们使用免疫组织化学和定量实时聚合酶链反应分析检测了乳腺癌组织和配对的正常组织中KIF15的表达，并对KIF15的表达与临床病理参数的相关性进行了统计学评估。在体内和体外研究了KIF15在乳腺癌细胞的增殖，迁移，肿瘤生长和转移中的作用，并且我们通过Western blot分析探索了KIF15在乳腺癌中潜在作用的潜在分子机制。结果表明，乳腺癌组织中KIF15表达的增加与肿瘤的大小，淋巴结转移和TNM分期呈正相关，而较高的KIF15表达则预示了乳腺癌患者的预后较差。此外，KIF15敲低显着减弱了乳腺癌细胞的体外，体内增殖，迁移，肿瘤生长和转移，沉默的KIF15表达显着抑制了磷酸化AKT，磷酸化JNK和细胞周期蛋白D1的表达，而p53和p21蛋白的表达均被抑制。大大增强。这些结果表明，KIF15是人乳腺癌中潜在的癌基因。KIF15的敲低显着减弱了乳腺癌细胞的体外，体内增殖，迁移，肿瘤生长和转移，沉默的KIF15表达显着抑制了磷酸化AKT，磷酸化的JNK和细胞周期蛋白D1的表达，而p53和p21蛋白的表达均大大增强了。 。这些结果表明，KIF15是人乳腺癌中潜在的癌基因。KIF15的敲低显着减弱了乳腺癌细胞的体外，体内增殖，迁移，肿瘤生长和转移，沉默的KIF15表达显着抑制了磷酸化AKT，磷酸化的JNK和细胞周期蛋白D1的表达，而p53和p21蛋白的表达均大大增强了。 。这些结果表明，KIF15是人乳腺癌中潜在的癌基因。