In order to determine the genetic differences between high-molecular weight glutenin subunits (HMW-GS) 7OE + 8* and 17 + 18, HMW-GS 7OE + 8* from a Canadian extra-strong wheat cultivar Glenlea was introduced to Longmai 20 (1, 17 + 18, 5 + 10) by 6 consecutive backcrosses with biochemical marker-assisted selection. The near-isogenic lines (NILs) of Longmai 20 possessing HMW-GS 7OE + 8* and 17 + 18 were grown in the experimental field of the Crop Breeding Institute of Heilongjiang Academy of Agricultural Sciences from 2006 to 2009. The field experiments were conducted in a two-column contrast arrangement with four replicates. Compared with the NILs possessing subunits 17 + 18, the quality parameters of NILs with subunits 7OE + 8*, which include protein content, dry gluten content, ratio of wet gluten/dry gluten, ratio of Zeleny sedimentation/dry gluten, stability, maximum resistance and area of extensogram, increased by 1.5% (P = 0.06), − 0.7% (P = 0.47), − 1.9% (P < 0.01), 6.6% (P = 0.02), 11.2% (P = 0.6), 20.3% (P < 0.01) and 19.9% (P < 0.01), respectively. HMW-GS 7OE + 8* have a greater positive effect on the rheological properties of flour, such as the maximum resistance and area of extensogram, which are less influenced by environmental conditions compared to stability of Farinogram.

中文翻译：

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HMW-GS 7OE+8*和17+18小麦品种龙麦20的NILs品质差异

为了确定高分子量麦谷蛋白亚基（HMW-GS）7OE+8*和17+18之间的遗传差异，将加拿大特强小麦品种Glenlea的HMW-GS 7OE+8*引入龙麦20（ 1, 17 + 18, 5 + 10) 通过 6 次连续回交与生化标记辅助选择。2006-2009年在黑龙江省农业科学院作物育种研究所试验田培育了具有HMW-GS 7OE+8*和17+18的龙麦20近等基因系（NILs）。在具有四个重复的两列对比排列中。与亚基17+18的NILs相比，亚基7OE+8*的NILs的质量参数包括蛋白质含量、干面筋含量、湿面筋/干面筋比例、Zeleny 沉降/干面筋比率、稳定性、最大阻力和拉伸图面积，增加 1.5% (P = 0.06), − 0.7% (P = 0.47), − 1.9% (P < 0.01), 6.6% (P = 0.02)、11.2% (P = 0.6)、20.3% (P < 0.01) 和 19.9% (P < 0.01)。HMW-GS 7OE + 8* 对面粉的流变特性有更大的积极影响，例如最大阻力和拉伸图的面积，与粉状图的稳定性相比，它们受环境条件的影响较小。