To study gene action and inheritance of some biochemical traits generation mean and generation variance analyses of basic seven generations B73 (as maternal line), MO17 (as paternal line), F₁, F₂, F_3, BC₁, and BC₂ were conducted at the University of Tabriz agricultural research station (NW-Iran) in 2017. The generations were sown in PVC pipes using a RCBD with 20 replications under three water regimes (100% FC, 55% and 75% available water depletion). The results revealed that based on percent increase of polyphenol oxidase, soluble sugars and proline, SC704 and MO17 were ranked as resistant (enhancement 41.39%, 58.39% and 55.47% in SC704 and 34.25%, 53.75% and 64.51% in MO17, respectively) under severe stress condition. Pearson’s correlation and principal component analysis indicated polyphenol oxidase activity most correlated with proline and soluble sugars and suggest that the important role of these traits in antioxidative defense mechanisms. The findings of the generation mean analysis implied the existence of digenic interactions. The prevalence of dominance variance and low narrow-sense heritability (0.07–0.47) for catalase, peroxidases, and polyphenol oxidase activities under severe stress condition suggest the utilization of a hybrid breeding program such as heterosis and selection in the later generation to facilitate breeding for improvement of these traits. In contrast, additive genetic variance and high narrow-sense heritability (0.53–0.71) for protein content, proline concentration and soluble sugars under severe stress condition revealed that the selection in the parents’ inbred lines or early segregating generations could be useful to improve the aforementioned traits in the maize.

研究基本的7代B73（作为母系），MO17（作为父系），F ₁，F ₂，F _3， BC ₁和BC _2的基因作用和某些生化性状的世代平均值和世代方差分析于2017年在大不里士大学农业研究站（NW-伊朗）进行。这些世代使用RCBD播种在PVC管中，并在三种水情下（100％FC，55％和75％可用水枯竭）重复20次。结果表明，基于多酚氧化酶，可溶性糖和脯氨酸的增加百分比，SC704和MO17被列为抗药性（SC704分别提高41.39％，58.39％和55.47％，MO17分别提高34.25％，53.75％和64.51％）。在严重的压力条件下。皮尔森（Pearson）的相关性和主成分分析表明，多酚氧化酶活性与脯氨酸和可溶性糖最相关，并表明这些性状在抗氧化防御机制中的重要作用。世代均值分析的发现暗示了双基因相互作用的存在。在严重胁迫条件下，过氧化氢酶，过氧化物酶和多酚氧化酶活性的优势变异和窄义遗传性较低（0.07-0.47），这表明在后代利用杂种育种和杂种优势进行选育有助于杂种的育种。这些特征的改善。相反，在严重胁迫条件下，蛋白质含量，脯氨酸浓度和可溶性糖的加性遗传方差和高窄义遗传力（0.53-0.71）表明，在亲本近交系或早期分离世代中进行选择可能有助于提高玉米的上述性状。重度胁迫条件下的多酚氧化酶活性和多酚氧化酶活性表明在后代利用杂种育种程序，例如杂种优势和选择，以促进育种以改善这些性状。相反，在严重胁迫条件下，蛋白质含量，脯氨酸浓度和可溶性糖的加性遗传方差和高窄义遗传力（0.53-0.71）表明，在亲本近交系或早期分离世代中进行选择可能有助于提高玉米的上述性状。重度胁迫条件下的多酚氧化酶活性和多酚氧化酶活性表明在后代利用杂种育种程序，例如杂种优势和选择，以促进育种以改善这些性状。相反，在严重胁迫条件下，蛋白质含量，脯氨酸浓度和可溶性糖的加性遗传方差和高窄义遗传力（0.53-0.71）表明，在亲本近交系或早期分离世代中进行选择可能有助于提高玉米的上述性状。