Isocoumarin formation by heterologous gene expression and modification by host enzymes.,Organic & Biomolecular Chemistry

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Isocoumarin formation by heterologous gene expression and modification by host enzymes.
Organic & Biomolecular Chemistry ( IF 2.9 ) Pub Date : 2020-06-22 , DOI: 10.1039/d0ob00989j
Pan Xiang ₁ , Lena Ludwig-Radtke ₁ , Wen-Bing Yin ₂ , Shu-Ming Li ₁

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Heterologous expression has been proven to be a successful strategy for the identification of metabolites encoded by cryptic/silent genes. Expression of a nonreducing polyketide synthase (NR-PKS) gene from Penicillium crustosum in Aspergillus nidulans led to the accumulation of three isocoumarins 1–3. Feeding experiments revealed that the PKS product 1 can be converted by the host enzymes to its hydroxylated (2) and methylated (3) derivatives. These results provided one additional example that unexpected further modifications of an enzyme product can take place in a heterologous host.

中文翻译：

异源香豆素通过异源基因表达形成，并通过宿主酶修饰。

异源表达已被证明是鉴定隐秘/沉默基因编码的代谢物的成功策略。在构巢曲霉中来自青霉青霉的非还原性聚酮化合物合酶（NR-PKS）基因的表达导致三个异香豆素1-3的积累。进料实验表明，PKS产物1可以被宿主酶转化为其羟基化（2）和甲基化（3）衍生物。这些结果提供了另一个实例，表明异源宿主中可能发生酶产物的意外进一步修饰。

更新日期：2020-07-08

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