A sensitive upconverting nanoprobe based on signal amplification technology for real-time in situ monitoring of drug-induced liver injury.,Nanoscale

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A sensitive upconverting nanoprobe based on signal amplification technology for real-time in situ monitoring of drug-induced liver injury.
Nanoscale ( IF 5.8 ) Pub Date : 2020-06-22 , DOI: 10.1039/d0nr01493a
Lingchang Meng ₁ , Xian Zheng ₁ , Zuguo Zheng ₁ , Zhen Zhao ₁ , Lai Wang ₁ , Ping Zhou ₁ , Gui-Zhong Xin ₁ , Ping Li ₁ , Hui-Jun Li ₁

Affiliation

Drug-induced liver injury (DILI) is increasingly recognized as one of the most challenging global health problems. Conventional in vitro detection methods not only lack specificity and sensitivity but also cannot achieve real-time, straightforward visualization of hepatotoxicity in vivo. Liver-specific miR122 has been observed to be a superior and sensitive biomarker for DILI diagnosis. Herein, a sensitive upconverting nanoprobe synthesized with upconversion nanoparticles (UCNPs) and gold nanorods (GNR) was designed to diagnose hepatotoxicity in vivo. After injection, the nanoprobes accumulated in the liver and were activated by miR122, and the signal amplification technology fully yielded luminescent amplification; hence, the detection sensitivity was improved. Because of the high tissue penetration capability of near-infrared light, this nanoprobe can achieve real-time in situ detection, thereby providing a novel technology for precise biological and medical analysis.

中文翻译：

基于信号放大技术的灵敏的上转换纳米探针，用于实时原位监测药物诱发的肝损伤。

药物引起的肝损伤（DILI）日益被认为是最具挑战性的全球健康问题之一。常规的体外检测方法不仅缺乏特异性和敏感性，而且无法实现体内肝毒性的实时，直接可视化。已观察到肝脏特异性miR122是DILI诊断的优良且敏感的生物标志物。本文中，设计了一种由上转换纳米颗粒（UCNPs）和金纳米棒（GNR）合成的敏感的上转换纳米探针，以诊断体内肝毒性。注射后，纳米探针在肝脏中积累并被miR122激活，信号放大技术完全产生了发光放大。因此，提高了检测灵敏度。由于近红外光具有很高的组织穿透能力，因此该纳米探针可以实现实时原位检测，从而为精确的生物学和医学分析提供了一种新颖的技术。

更新日期：2020-07-23

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