Identifying abnormalities in red blood cells can provide important medical clues for the diagnosis, prognosis, and treatment of some health disorders. A common test used to examine the conditions of erythrocytes in humans is osmotic fragility. The standard technique to determine the osmotic fragility of red blood cells is laborious and time-consuming, and provides only approximate values with a few experimental data. In this work, we propose and investigate a way to measure the osmotic fragility of erythrocytes, rapidly and in a straightforward, more quantitative and less laborious way, with an optical sensor. The basic idea is monitoring in real time the refractive index of a suspension of erythrocytes to follow the kinetics of their lysis as the hemoglobin-rich cytosol is released from the erythrocytes in the surrounding hypotonic solution during hemolysis, resulting in an increase of the refractive index. We propose a device to measure in real time the refractive index and de...

中文翻译：

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用于低渗介质中溶血的光学传感的光学装置和方法

识别红细胞异常可以为某些健康疾病的诊断，预后和治疗提供重要的医学线索。用于检查人体红细胞状况的常见测试是渗透性脆弱性。确定红细胞渗透性脆性的标准技术既费力又费时，并且仅提供一些实验数据的近似值。在这项工作中，我们提出并研究了一种使用光学传感器以快速，直接，定量，省力的方式测量红细胞的渗透脆性的方法。基本思想是实时监测红细胞悬液的折射率，以跟随其溶解的动力学，因为溶血过程中富血红蛋白的细胞溶质从周围低渗溶液中的红细胞中释放出来，导致折射率增加。我们提出一种可以实时测量折射率和折射率的装置。