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Rapid and selective detection of recombinant human erythropoietin in human blood plasma by a sensitive optical sensor.
Analyst ( IF 3.6 ) Pub Date : 2020-06-22 , DOI: 10.1039/d0an00972e Mahnaz D Gholami 1 , Frederick Theiss 1 , Prashant Sonar 2 , Godwin A Ayoko 2 , Emad L Izake 2
Analyst ( IF 3.6 ) Pub Date : 2020-06-22 , DOI: 10.1039/d0an00972e Mahnaz D Gholami 1 , Frederick Theiss 1 , Prashant Sonar 2 , Godwin A Ayoko 2 , Emad L Izake 2
Affiliation
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Recombinant human erythropoietin (rHuEPO) is an important hormone drug that is used to treat several medical conditions. It is also frequently abused by athletes as a performance enhancing agent at sporting events. The time window of the rHuEPO in blood is short. Therefore, the rapid detection of rHuEPO use/abuse at points of care and in sports requires a selective analytical method and a sensitive sensor. Herein, we present a highly selective method for the rapid detection of rHuEPO in human blood plasma by a sensitive optical sensor. rHuEPO is selectively extracted from human blood plasma by a target-specific extractor chip and converted into a biothiol by reducing its disulfide bond structure. The formed biothiol reacts with a water soluble (E)-1-((6-methoxybenzo[d]thiazole-2-yl)diazenyl)naphthalene-2,6-diolHg(II) (BAN-Hg) optical sensor and causes its rapid decomposition. This leads to a rapid change in the sensor color from blue to pink that can be observed by the naked eye. The optical sensor was used to quantify rHuEPO in the concentration range 1 × 10−8 M to 1 × 10−12 M by UV–Vis spectroscopy. For the screening of blood plasma, an EPO-specific extractor chip was synthesized and used to selectively extract the protein from the biological matrix prior to its conversion into biothiol and quantification by the optical sensor. Since many proteins have a disulfide bond structure, the new method has strong potential for their rapid sensitive and selective detection by the BAN-Hg sensor and UV–Vis spectroscopy.
中文翻译：
通过灵敏的光学传感器快速，选择性地检测人血浆中的重组人促红细胞生成素。
重组人促红细胞生成素（rHuEPO）是一种重要的激素药物，可用于治疗多种疾病。在体育比赛中，它也经常被运动员滥用为性能增强剂。血液中的rHuEPO的时间窗口很短。因此，在护理点和运动中快速检测rHuEPO的使用/滥用需要选择性的分析方法和灵敏的传感器。在这里，我们提出了一种高度灵敏的方法，用于通过灵敏的光学传感器快速检测人血浆中的rHuEPO。通过靶标特异性提取芯片选择性地从人血浆中提取rHuEPO，并通过还原其二硫键结构将其转化为生物硫醇。形成的生物硫醇与水溶性（E）-1-（（6-6-甲氧基苯并[ d]噻唑-2-基）二氮烯基）萘-2,6-二醇Hg （II）（BAN-Hg）光学传感器并引起其快速分解。这导致肉眼可观察到的传感器颜色从蓝色快速变为粉红色。通过紫外-可见光谱法，使用光学传感器对浓度为1×10 -8 M至1×10 -12 M的rHuEPO进行定量。为了筛选血浆，合成了EPO特异性提取芯片，并用于从生物基质中选择性提取蛋白质，然后将其转化为生物硫醇并通过光学传感器进行定量。由于许多蛋白质具有二硫键结构，因此该新方法具有通过BAN-Hg传感器和UV-Vis光谱法进行快速灵敏和选择性检测的强大潜力。
更新日期：2020-08-10
中文翻译：
通过灵敏的光学传感器快速，选择性地检测人血浆中的重组人促红细胞生成素。
重组人促红细胞生成素（rHuEPO）是一种重要的激素药物，可用于治疗多种疾病。在体育比赛中，它也经常被运动员滥用为性能增强剂。血液中的rHuEPO的时间窗口很短。因此，在护理点和运动中快速检测rHuEPO的使用/滥用需要选择性的分析方法和灵敏的传感器。在这里，我们提出了一种高度灵敏的方法，用于通过灵敏的光学传感器快速检测人血浆中的rHuEPO。通过靶标特异性提取芯片选择性地从人血浆中提取rHuEPO，并通过还原其二硫键结构将其转化为生物硫醇。形成的生物硫醇与水溶性（E）-1-（（6-6-甲氧基苯并[ d]噻唑-2-基）二氮烯基）萘-2,6-二醇Hg （II）（BAN-Hg）光学传感器并引起其快速分解。这导致肉眼可观察到的传感器颜色从蓝色快速变为粉红色。通过紫外-可见光谱法，使用光学传感器对浓度为1×10 -8 M至1×10 -12 M的rHuEPO进行定量。为了筛选血浆，合成了EPO特异性提取芯片，并用于从生物基质中选择性提取蛋白质，然后将其转化为生物硫醇并通过光学传感器进行定量。由于许多蛋白质具有二硫键结构，因此该新方法具有通过BAN-Hg传感器和UV-Vis光谱法进行快速灵敏和选择性检测的强大潜力。
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