Oral health and dentistry are essential components of systems medicine, which has received lesser attention in comparison to other medical fields, such as cancer biology. In this context, oral polymorphonuclear neutrophils (oPMNs) play an important role in the maintenance of oral health. To the best of our knowledge, this is the first study to report original observations on the transcriptional responses of oPMNs during experimentally induced gingivitis, by temporarily refraining from regular oral care. Oral rinses were prospectively collected at four different time points for oPMNs isolation from healthy volunteers: day 1 (start of the experimental gingivitis challenge), day 9 (during challenge), day 14 (end of the challenge), and day 21 (postchallenge). Transcriptome of oPMNs was determined by RNA sequencing. Differentially expressed genes (DEGs) were selected at p < 0.01 level, and evaluated for pathway regulation using Ingenuity Pathway Analysis suite. We found four major clusters of DEGs, consisting of 256 initial response DEGs (day 9 only), 221 late response DEGs (day 14 only), 53 persistent responsive DEGs (consistent at day 9 and 14), and 524 DEGs showing responses only in the postchallenge phase (day 21 only). Pathway analysis of the initial and late response DEGs showed involvement in many immune regulatory pathways and PMN function, whereas DEGs at day 21 were associated with epithelial adherence signaling and other miscellaneous related signaling pathways. The results from this pilot study showed that oPMNs mediate oral inflammatory processes, suggesting their immunomodulatory role in oral equilibrium.

中文翻译：

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牙科和 OMICS：通过实验性牙龈炎中口腔多形核中性粒细胞的变化测量的口腔生态系统的转录组动力学。

口腔健康和牙科是系统医学的重要组成部分，与癌症生物学等其他医学领域相比，系统医学受到的关注较少。在这种情况下，口腔多形核中性粒细胞 (oPMNs) 在维持口腔健康方面发挥着重要作用。据我们所知，这是第一项通过暂时避免定期口腔护理来报告对实验性牙龈炎期间 oPMNs 转录反应的原始观察的研究。在四个不同时间点前瞻性收集口腔冲洗液，用于从健康志愿者中分离 oPMN：第 1 天（实验性牙龈炎攻击开始）、第 9 天（攻击期间）、第 14 天（攻击结束）和第 21 天（攻击后） . oPMNs 的转录组由 RNA 测序确定。p  < 0.01 水平，并使用 Ingenuity Pathway Analysis 套件评估通路调节。我们发现了四个主要的 DEG 集群，包括 256 个初始响应 DEG（仅第 9 天）、221 个延迟响应 DEG（仅第 14 天）、53 个持续响应 DEG（在第 9 天和第 14 天一致）和 524 个仅显示响应的 DEG挑战后阶段（仅限第 21 天）。初始和晚期反应 DEG 的通路分析显示参与许多免疫调节通路和 PMN 功能，而第 21 天的 DEG 与上皮粘附信号和其他杂项相关信号通路相关。这项初步研究的结果表明，oPMNs 介导口腔炎症过程，表明它们在口腔平衡中具有免疫调节作用。