当前位置：
X-MOL 学术
›
Biomacromolecules
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Stabilizing Enzymes within Polymersomes by Coencapsulation of Trehalose.
Biomacromolecules ( IF 5.5 ) Pub Date : 2020-06-22 , DOI: 10.1021/acs.biomac.0c00824 Maria Valentina Dinu 1, 2 , Ionel Adrian Dinu 1, 2 , Sina S Saxer 3 , Wolfgang Meier 1 , Uwe Pieles 3 , Nico Bruns 4, 5
Biomacromolecules ( IF 5.5 ) Pub Date : 2020-06-22 , DOI: 10.1021/acs.biomac.0c00824 Maria Valentina Dinu 1, 2 , Ionel Adrian Dinu 1, 2 , Sina S Saxer 3 , Wolfgang Meier 1 , Uwe Pieles 3 , Nico Bruns 4, 5
Affiliation
|
Enzymes are essential biocatalysts and very attractive as therapeutics. However, their functionality is strictly related to their stability, which is significantly affected by the environmental changes occurring during their usage or long-term storage. Therefore, maintaining the activity of enzymes is essential when they are exposed to high temperature during usage or when they are stored for extended periods of time. Here, we stabilize and protect enzymes by coencapsulating them with trehalose into polymersomes. The anhydrobiotic disaccharide preserved up to about 81% of the enzyme’s original activity when laccase/trehalose-loaded nanoreactors were kept desiccated for 2 months at room temperature and 75% of its activity when heated at 50 °C for 3 weeks. Moreover, the applicability of laccase/trehalose-loaded nanoreactors as catalysts for bleaching of the textile dyes orange G, toluidine blue O, and indigo was proven. Our results demonstrate the advantages of coencapsulating trehalose within polymersomes to stabilize enzymes in dehydrated state for extended periods of time, preserving their activity even when heated to elevated temperature.
中文翻译：
通过共封装海藻糖来稳定多聚体中的酶。
酶是必不可少的生物催化剂，作为治疗剂非常有吸引力。但是，它们的功能与它们的稳定性严格相关，这在使用或长期存储期间会受到环境变化的严重影响。因此，当酶在使用过程中暴露于高温或长时间保存时，维持酶的活性至关重要。在这里，我们通过将酶与海藻糖共包封到多聚体中来稳定和保护酶。当将负载有漆酶/海藻糖的纳米反应器在室温下干燥2个月时，该无水生二糖最多保留了该酶原始活性的81％，而在50°C下加热3周则其活性的75％保留了该酶的原始活性。此外，负载漆酶/海藻糖的纳米反应器作为催化剂可用于纺织染料橙色G，甲苯胺蓝O和靛蓝的漂白。我们的结果证明了将海藻糖共包封在聚合物囊泡中的优势，可以使酶长时间处于脱水状态，即使在加热到高温时也能保持其活性。
更新日期：2020-06-22
中文翻译：
通过共封装海藻糖来稳定多聚体中的酶。
酶是必不可少的生物催化剂，作为治疗剂非常有吸引力。但是，它们的功能与它们的稳定性严格相关，这在使用或长期存储期间会受到环境变化的严重影响。因此，当酶在使用过程中暴露于高温或长时间保存时，维持酶的活性至关重要。在这里，我们通过将酶与海藻糖共包封到多聚体中来稳定和保护酶。当将负载有漆酶/海藻糖的纳米反应器在室温下干燥2个月时，该无水生二糖最多保留了该酶原始活性的81％，而在50°C下加热3周则其活性的75％保留了该酶的原始活性。此外，负载漆酶/海藻糖的纳米反应器作为催化剂可用于纺织染料橙色G，甲苯胺蓝O和靛蓝的漂白。我们的结果证明了将海藻糖共包封在聚合物囊泡中的优势，可以使酶长时间处于脱水状态，即使在加热到高温时也能保持其活性。
京公网安备 11010802027423号