Imaging of DNA and RNA in Living Eukaryotic Cells to Reveal Spatiotemporal Dynamics of Gene Expression.,Annual Review of Biochemistry

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Imaging of DNA and RNA in Living Eukaryotic Cells to Reveal Spatiotemporal Dynamics of Gene Expression.
Annual Review of Biochemistry ( IF 12.1 ) Pub Date : 2020-06-22 , DOI: 10.1146/annurev-biochem-011520-104955
Hanae Sato ₁ , Sulagna Das ₁ , Robert H Singer _{1,

2} , Maria Vera _{1,

3}

Affiliation

This review focuses on imaging DNA and single RNA molecules in living cells to define eukaryotic functional organization and dynamic processes. The latest advances in technologies to visualize individual DNA loci and RNAs in real time are discussed. Single-molecule fluorescence microscopy provides the spatial and temporal resolution to reveal mechanisms regulating fundamental cell functions. Novel insights into the regulation of nuclear architecture, transcription, posttranscriptional RNA processing, and RNA localization provided by multicolor fluorescence microscopy are reviewed. A perspective on the future use of live imaging technologies and overcoming their current limitations is provided.

中文翻译：

活的真核细胞中DNA和RNA的成像，揭示了基因表达的时空动态。

这篇综述着重于对活细胞中的DNA和单个RNA分子进行成像，以定义真核功能组织和动态过程。讨论了实时可视化单个DNA基因座和RNA的技术的最新进展。单分子荧光显微镜提供了时空分辨率，揭示了调节基本细胞功能的机制。综述了多色荧光显微镜对核结构，转录，转录后RNA加工和RNA定位调控的新见解。提供了有关实时成像技术的未来用途以及如何克服其当前局限性的观点。

更新日期：2020-06-23

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