The insect commensal microbiota consists of prokaryotes and eukaryotes. We explored the effect of diet and the persistence of the gut microbiota across generations in Drosophila suzukii (Matsumura) (Diptera: Drosophilidae). We transferred subsets of a single population of D. suzukii to different fruit-based diets (blueberry (Vaccinium Linnaeus; Ericaceae), raspberry (Rubus Linnaeus; Rosaceae), and strawberry (Fragaria × ananassa Duchesne; Rosaceae)) for three generations and then returned them to a common, banana-based, diet. We used 16S rDNA (Bacteria) and ITS (internal transcribed spacer; Fungi) sequencing of female endosymbiont-free flies to identify the microbiota. We identified 2700 bacterial and 350 fungal operational taxonomic units (OTUs); there was no correlation between the number of bacterial and fungal OTUs in a sample. Bacterial communities were dominated by Proteobacteria (especially Acetobacteraceae); Ascomycota dominated the fungal communities. Species diversity of both bacteria and fungi differed among diets, but there were no differences in species-level diversity when these D. suzukii were returned to a control diet. A principle coordinates analysis revealed no differences in the bacterial or fungal community in the first generation on fruit diets, but that the communities diverged over the next two generations; neither fungal and bacterial communities converged after one generation on control food. We conclude that diet changes the D. suzukii microbiota, and that these changes persist for more than one generation.

昆虫共生菌群由原核生物和真核生物组成。我们探讨了饮食的影响以及铃木果蝇（松村）（双翅目：果蝇科）中不同世代的肠道菌群的持久性。我们转移的单个群体的子集D. suzukii不同基于水果的食物（蓝莓（越桔Linnaeus的;杜鹃花科），覆盆子（悬钩子Linnaeus的;蔷薇科）和草莓（草莓× ananassaDuchesne; 蔷薇科）三代，然后将它们恢复为以香蕉为基础的普通饮食。我们使用雌性无内共生体蝇的16S rDNA（细菌）和ITS（内部转录间隔子；真菌）测序来鉴定微生物群。我们确定了2700个细菌和350个真菌操作分类单位（OTU）；样品中细菌和真菌OTU的数量之间没有相关性。细菌群落以变形杆菌（尤其是醋杆菌科）为主；子囊菌在真菌群落中占主导地位。饮食中细菌和真菌的物种多样性都不同，但是当这些铃木D. suzukii时，物种水平的多样性没有差异。恢复控制饮食。一次主坐标分析表明，在水果饮食的第一代中，细菌或真菌群落没有差异，但在随后的两代中，细菌或真菌的群落有所不同。在控制食物上世代相传后，真菌和细菌群落都没有融合。我们得出的结论是，饮食改变了铃木D.菌群，并且这些改变持续了超过一代。