Solid Composite Material for Delivering Viable Cells into Skin Tissues via Detachable Dissolvable Microneedles,ACS Applied Bio Materials

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Solid Composite Material for Delivering Viable Cells into Skin Tissues via Detachable Dissolvable Microneedles
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-06-21 , DOI: 10.1021/acsabm.0c00498
Puttikorn Pukfukdee ₁ , Wijit Banlunara ₂ , Teeranut Rutwaree ₃ , Benchaphorn Limcharoen ₄ , Pritsana Sawutdeechaikul ₁ , Thitiporn Pattarakankul ₅ , Titiporn Sansureerungsikul ₃ , Pattrawadee Toprangkobsin ₆ , Asada Leelahavanichkul ₇ , Ratchathorn Panchaprateep ₈ , Pravit Asawanonda ₈ , Tanapat Palaga ₉ , Supason Wanichwecharungruang ₁

Affiliation

Delivering cells to desired locations in the body is needed for disease treatments, tissue repairs, and various scientific investigations such as animal models for drug development. Here, we report the solid composite material that when embedded with viable cells, can temporarily keep cells alive. Using the material, we also show the fabrication of detachable dissolvable microneedles (DMNs) that can instantly deliver viable cells into skin tissue. B16-F10-murine-melanoma (B16-F10) and human-embryonic-kidney-293T (HEK293T) cells embedded in the solid matrix of the hyaluronic/polyvinylpyrolidone/maltose (HA/PVP/maltose) mixture show 50.6 ± 12.0 and 71.0 ± 5.96% survivals, respectively, when kept at 4 °C for 24 h. Detachable DMNs made of the HA/PVP/maltose mixture and loaded with B16-F10-cells were constructed, and the obtained DMN patches could detach the cell-loaded needles into the skin within 1 min of patch application. In vivo intradermal tumorgrafting mice with the DMNs containing 800 cells of B16-F10 developed tumors 10 times bigger in volume than tumors induced by hypodermic needle injection of suspension containing 100,000 cells. We anticipate this work to be a starting point for viable cell encapsulation in the solid matrix and viable cell delivery via DMNs.

中文翻译：

用于通过可拆卸可溶解微针将活细胞输送到皮肤组织中的固体复合材料

疾病治疗、组织修复和各种科学研究（例如用于药物开发的动物模型）都需要将细胞输送到体内所需位置。在这里，我们报告了一种固体复合材料，当嵌入活细胞时，可以暂时保持细胞存活。使用该材料，我们还展示了可拆卸可溶解微针 (DMN) 的制造，该微针可以立即将活细胞输送到皮肤组织中。嵌入透明质酸/聚乙烯吡咯烷酮/麦芽糖 (HA/PVP/麦芽糖) 混合物的固体基质中的 B16-F10-鼠-黑色素瘤 (B16-F10) 和人-胚胎-肾脏-293T (HEK293T) 细胞显示 50.6 ± 12.0 和 71.0 ± 5.96% 的存活率分别在 4 °C 下保持 24 小时。构建了由 HA/PVP/麦芽糖混合物制成并装载 B16-F10 细胞的可拆卸 DMN，使用含有 800 个 B16-F10 细胞的 DMN 进行体内皮内肿瘤移植小鼠，其肿瘤体积比皮下注射针头注射含有 100,000 个细胞的悬浮液诱导的肿瘤体积大 10 倍。我们预计这项工作将成为固体基质中的活细胞封装和通过DMN 传递活细胞的起点。

更新日期：2020-07-20

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