Abstract In this study, an amidase-synthesizing and inducible thermotolerant bacterium, Bacillus tequilensis (BITNR004), was investigated for the conversion of acrylamide to acrylic acid, which was subsequently confirmed using various analytical tools. The enzyme, acrylamidase, was found to be intracellular in nature with optimum activity at 50 °C. Further, the whole-cell biocatalyst had a biotransformation efficiency of 63% and 59% at 1 mM and 2 mM acrylamide, respectively, as determined via high performance liquid chromatography, while acrylamide-supplemented synthesis medium only had 13% biotransformation efficiency. The substrate and product were detected via thin-layer chromatography, using methanol and water (3.5:1.5 v/v) as mobile phase. Fourier-transform infra-red spectra revealed the presence of the C = C stretch of the alkenyl group of acrylic acid at the frequency range of 1630–1675 cm−1 and of carboxylate at 1390–1440 cm−1, confirming the bioconversion of acrylamide to acrylic acid. Based on whole-cell catalysis, B. tequilensis (BITNR004) can be exploited for the production of acrylic acid and bioremediation of acrylamide-contaminated waste water.

中文翻译：

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通过龙舌兰芽孢杆菌全细胞合成的丙烯酰胺酶将丙烯酰胺生物转化为丙烯酸 (BITNR004)

摘要 在这项研究中，研究了一种合成酰胺酶和诱导型耐热细菌龙舌兰芽孢杆菌 (BITNR004) 将丙烯酰胺转化为丙烯酸，随后使用各种分析工具对其进行了确认。发现酶丙烯酰胺酶本质上是细胞内的，在 50°C 时具有最佳活性。此外，通过高效液相色谱法测定，全细胞生物催化剂在 1 mM 和 2 mM 丙烯酰胺时的生物转化效率分别为 63% 和 59%，而添加丙烯酰胺的合成培养基仅具有 13% 的生物转化效率。通过薄层色谱法检测底物和产物，使用甲醇和水 (3.5:1.5 v/v) 作为流动相。傅里叶变换红外光谱揭示了在 1630-1675 cm-1 频率范围内丙烯酸的烯基和 1390-1440 cm-1 频率范围内存在 C = C 拉伸，证实了丙烯酰胺的生物转化到丙烯酸。基于全细胞催化，龙舌兰酒 (BITNR004) 可用于生产丙烯酸和生物修复丙烯酰胺污染的废水。