ABSTRACT Cell cultures established from various plant species have been used for a range of physiological and biochemical studies. Homogeneity of cell types and size of clusters in the cell culture often gave a clearer and simpler results compared to those obtained with the whole plant. On the other hand, possible variability of physiological conditions and responsiveness to external stimuli between the cell lines could be problematic for comparative studies. Aiming at combining the usefulness of plant cell culture with the rich information and genetic resources of Arabidopsis, we systemically examined the methods/conditions to establish cell lines for comparative studies, which could be applicable to a variety of genetic resources. Arabidopsis cell lines thus established from the meristem of mature seeds showed reproducible and comparable MAMP responses such as ROS generation and defense-related gene expression. MAMP responses of the cultured cells showed the specificity depending on the presence/absence of the corresponding MAMP receptor. Pharmacological study with a protein kinase inhibitor, K252a, also showed the usefulness of the cell culture for such studies. These results indicated the usefulness of the method to establish Arabidopsis cell lines, which are useful for comparative studies between genetic resources.

中文翻译：

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拟南芥细胞培养用于可比较的生理和遗传研究

摘要从各种植物物种建立的细胞培养物已用于一系列生理和生化研究。与用整株植物获得的结果相比，细胞培养物中细胞类型和簇大小的同质性通常会给出更清晰、更简单的结果。另一方面，生理条件的可能变异性和细胞系之间对外部刺激的反应性可能会给比较研究带来问题。为了将植物细胞培养的实用性与拟南芥丰富的信息和遗传资源相结合，我们系统地研究了建立细胞系的方法/条件进行比较研究，可适用于多种遗传资源。因此从成熟种子的分生组织建立的拟南芥细胞系显示出可重复和可比较的 MAMP 反应，例如 ROS 生成和防御相关基因表达。培养细胞的 MAMP 反应表现出特异性，取决于相应 MAMP 受体的存在/不存在。用蛋白激酶抑制剂 K252a 进行的药理学研究也显示了细胞培养对此类研究的有用性。这些结果表明该方法建立拟南芥细胞系的有用性，可用于遗传资源之间的比较研究。还显示了细胞培养对此类研究的有用性。这些结果表明该方法建立拟南芥细胞系的有用性，可用于遗传资源之间的比较研究。还显示了细胞培养对此类研究的有用性。这些结果表明该方法建立拟南芥细胞系的有用性，可用于遗传资源之间的比较研究。