Abstract

This experiment was aimed to study the effect of sugarcane bagasse (SB) fermented with Lactobacillus casei TH14, cellulase, and molasses on in vitro gas kinetics, nutrient digestibility, and ruminal fermentation patterns compared to rice straw (RS). A 2 × 2 × 2 (+1) factorial arrangement in a completely randomized design was used. Factor A was levels of L. casei TH14 at 0 and 0.05 g/kg fresh matter, factor B was levels of cellulase at 0 and 10⁴ U/kg fresh matter, and factor C was levels of molasses at 0 and 5 g of substrate DM. The (+1) treatment referred to RS fermentation without additives. The results showed that kinetics of gas, gas production, and total volatile fatty acid were not different between RS and SB treatment. RS had significantly (p < 0.05) greater nutrient digestibility, ruminal pH, ammonia-nitrogen, and protozoa compared the control SB. Compared with control RS and SB, SB fermented with additives had greater (p < 0.05) gas from soluble fraction and rate constant of gas, in vitro dry matter and neutral detergent fiber digestibility, and ruminal propionate. In conclusion, SB fermented with L. casei TH14, cellulase, and molasses in combination promoted ruminal gas production, in vitro digestibility, and ruminal fermentation patterns.

摘要

本实验旨在研究与稻草 (RS) 相比，用干酪乳杆菌TH14、纤维素酶和糖蜜发酵的甘蔗渣 (SB) 对体外气体动力学、养分消化率和瘤胃发酵模式的影响。使用完全随机设计中的 2 × 2 × 2 (+1) 因子排列。因子 A 是 0 和 0.05 g/kg 新鲜物质的干酪乳杆菌TH14 水平，因子 B 是 0 和 10 ^{4的纤维素酶水平}U/kg 新鲜物质和因子 C 是 0 和 5 g 底物 DM 时的糖蜜水平。(+1) 处理是指无添加剂的 RS 发酵。结果表明，RS 和 SB 处理的产气动力学、产气动力学和总挥发性脂肪酸没有差异。与对照 SB 相比， RS 具有显着 ( p  < 0.05) 更高的养分消化率、瘤胃 pH 值、氨氮和原生动物。与对照 RS 和 SB 相比，添加剂发酵 SB 的可溶性部分气体和气体速率常数、体外干物质和中性洗涤纤维消化率和瘤胃丙酸盐具有更高的 ( p < 0.05)。总之，SB与干酪乳杆菌发酵TH14、纤维素酶和糖蜜的组合促进了瘤胃产气、体外消化率和瘤胃发酵模式。