ABSTRACT Deltamethrin is a widely used pyrethroits worldwide. Although the chemical is used to combat insects, it has effects on other non-target organisms. Deltamethrin is extensively used in agriculture, animal husbandry, and domestic areas in the Lake Van basin. The aim of this study was to determine the cytotoxic, genotoxic, and oxidative damage from deltamethrin on the primary hepatocyte culture of Lake Van fish (Alburnus tarichi). In this study, the toxic effects of different concentrations (0.01, 0.1, 1, and 10 µM) of deltamethrin in the primary hepatocyte culture of Lake Van fish were investigated via liver enzymes aspartase aminotransferase (AST) and alanine aminotransferase (ALT), and the total antioxidant status (TAS), total oxidant status (TOS), lipid peroxidation (MDA), and DNA damage (8-OHdG). Deltamethrin caused an increase in the AST and ALT levels dependent on the dosage and time. The TAS and TOS levels increased at the end of 24 h and there was no difference at the end of 48 h. Deltamethrin did not affect the MDA level, but increased the 8-OHdG (P < 0.05). In conclusion, it can be said that high doses of deltamethrin (1 and 10 µM) have a toxic effect on the primary hepatocyte culture of Lake Van fish.

中文翻译：

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确定溴氰菊酯对凡湖鱼 (Alburnus tarichi) 原代肝细胞培养的细胞毒性、遗传毒性和氧化损伤

摘要 溴氰菊酯是世界范围内广泛使用的除虫菊酯。尽管该化学品用于对抗昆虫，但它对其他非目标生物也有影响。溴氰菊酯广泛用于万湖盆地的农业、畜牧业和家庭地区。本研究的目的是确定溴氰菊酯对凡湖鱼 (Alburnus tarichi) 原代肝细胞培养物的细胞毒性、基因毒性和氧化损伤。在本研究中，通过肝酶天冬氨酸转氨酶 (AST) 和丙氨酸转氨酶 (ALT) 研究了不同浓度（0.01、0.1、1 和 10 µM）溴氰菊酯在凡湖鱼原代肝细胞培养中的毒性作用，以及总抗氧化状态 (TAS)、总氧化状态 (TOS)、脂质过氧化 (MDA) 和 DNA 损伤 (8-OHdG)。溴氰菊酯导致 AST 和 ALT 水平升高，这取决于剂量和时间。TAS 和 TOS 水平在 24 小时结束时增加，在 48 小时结束时没有差异。溴氰菊酯不会影响 MDA 水平，但会增加 8-OHdG（P < 0.05）。总之，可以说高剂量的溴氰菊酯（1 和 10 µM）对凡湖鱼的原代肝细胞培养具有毒性作用。