Introduction The membrane-associated G protein-coupled estrogen receptor 1 (GPER) mediates the regulation by estradiol on arginine-vasopressin immunoreactivity in the supraoptic and paraventricular hypothalamic nuclei of female rats and is involved in the estrogenic control of hypothalamic regulated functions, such as food intake, sexual receptivity and lordosis behavior. Objective To assess GPER distribution in the rat hypothalamus. Methods GPER immunoreactivity was assessed in different anatomical subdivisions of five selected hypothalamic regions of young adult male and cycling female rats: the arcuate nucleus, the lateral hypothalamus, the paraventricular nucleus, the supraoptic nucleus and the ventromedial hypothalamic nucleus. GPER immunoreactivity was colocalized with NeuN as a marker of mature neurons, GFAP as a marker of astrocytes and CC1 as a marker of mature oligodendrocytes. Results GPER immunoreactivity was detected in hypothalamic neurons, astrocytes and oligodendrocytes. Sex and regional differences and changes during the estrous cycle were detected in the total number of GPER immunoreactive cells and in the proportion of neurons, astrocytes and oligodendrocytes that were GPER immunoreactive. Conclusions These findings suggest that estrogenic regulation of hypothalamic function through GPER may be different in males and in females and may fluctuate during the estrous cycle in females.


中文翻译：

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大鼠下丘脑中G蛋白偶联雌激素受体的免疫反应广泛分布于神经元、星形胶质细胞和少突胶质细胞中，在动情周期中波动，呈两性二态。

简介 膜相关 G 蛋白偶联雌激素受体 1 (GPER) 介导雌二醇对雌性大鼠视上和室旁下丘脑核精氨酸-加压素免疫反应性的调节，并参与下丘脑调节功能的雌激素控制，例如食物摄入量、性接受能力和前凸行为。目的评估大鼠下丘脑GPER分布。方法 在年轻成年雄性和骑自行车的雌性大鼠的五个选定下丘脑区域的不同解剖分区中评估 GPER 免疫反应性：弓状核、下丘脑外侧核、室旁核、视上核和下丘脑腹内侧核。GPER 免疫反应性与 NeuN 共定位，作为成熟神经元的标记，GFAP 作为星形胶质细胞的标志物，CC1 作为成熟少突胶质细胞的标志物。结果在下丘脑神经元、星形胶质细胞和少突胶质细胞中检测到GPER免疫反应。在发情周期中检测到 GPER 免疫反应细胞总数以及具有 GPER 免疫反应的神经元、星形胶质细胞和少突胶质细胞的比例的性别和区域差异和变化。结论 这些研究结果表明，雌激素通过 GPER 调节下丘脑功能在男性和女性中可能不同，并且可能在女性的发情周期中波动。在发情周期中检测到 GPER 免疫反应细胞总数以及具有 GPER 免疫反应的神经元、星形胶质细胞和少突胶质细胞的比例的性别和区域差异和变化。结论 这些研究结果表明，雌激素通过 GPER 调节下丘脑功能在男性和女性中可能不同，并且可能在女性的发情周期中波动。在发情周期中检测到 GPER 免疫反应细胞总数和具有 GPER 免疫反应的神经元、星形胶质细胞和少突胶质细胞的比例的性别和区域差异和变化。结论 这些研究结果表明，雌激素通过 GPER 调节下丘脑功能在男性和女性中可能不同，并且可能在女性的发情周期中波动。