Abstract Plant Growth Promoting Rhizobacteria (PGPR) influence plants’ physiological characteristics, metabolites, pathways and proteins via alteration of corresponding gene expression. In the current study, a total of 42 upregulated uncharacterized sorghum bicolor root proteins influenced by PGPR were subjected to different analyses: phylogenetic tree, protein functional network, sequences similarity network (SSN), Genome Neighborhood Network (GNN) and motif analysis. The screen for homologous bacterial proteins to uncover associated protein families and similar proteins in non-PGPRs was identified. The sorghum roots’ uncharacterized protein sequences analysis indicated the existence of two protein categories, the first being related to phytobeneficial protein family associated with DNA regulation such as Sulfatase, FGGY_C, Phosphodiesterase or stress tolerance such as HSP70. The second is associated with bacterial transcriptional regulators such as FtsZ, MreB_Mbl and DNA-binding transcriptional regulators, as well as the AcrR family, which existed in PGPR and non PGPR. Therefore, Plant Growth-Promoting Rhizobacteria (PGPR) regulated phytobeneficial traits through reciprocal protein stimulation via microbe plant interactions, both during and post colonization.

中文翻译：

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促进植物生长的根际细菌 (PGPR) 调节植物和微生物有益蛋白质的相互作用

摘要 植物促生长根际细菌(Plant Growth Promoting Rhizobacteria, PGPR) 通过改变相应的基因表达来影响植物的生理特性、代谢产物、途径和蛋白质。在目前的研究中，共有 42 种受 PGPR 影响的上调未表征的高粱双色根蛋白进行了不同的分析：系统发育树、蛋白质功能网络、序列相似性网络 (SSN)、基因组邻域网络 (GNN) 和基序分析。鉴定了同源细菌蛋白质的筛选，以揭示非 PGPR 中的相关蛋白质家族和类似蛋白质。高粱根未表征的蛋白质序列分析表明存在两种蛋白质类别，第一种与与 DNA 调控相关的植物有益蛋白家族有关，如硫酸脂肪酶、FGGY_C、磷酸二酯酶或胁迫耐受性，如 HSP70。第二个与细菌转录调节因子有关，例如 FtsZ、MreB_Mbl 和 DNA 结合转录调节因子，以及存在于 PGPR 和非 PGPR 中的 AcrR 家族。因此，在定植期间和定植后，植物生长促进根际细菌 (PGPR) 通过微生物植物相互作用的互惠蛋白质刺激来调节植物有益性状。