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In-house validation method for quantification of amoxicillin in medicated feedingstuffs with the use of HPLC-DAD technique
Journal of Veterinary Research ( IF 1.3 ) Pub Date : 2020-06-20 , DOI: 10.2478/jvetres-2020-0044
Ewelina Patyra 1 , Krzysztof Kwiatek 1
Affiliation  

Abstract Introduction A high-performance liquid chromatographic–diode array detector (HPLC-DAD) method for the determination of amoxicillin in medicated feedingstuffs was developed and validated. The method was used to investigate the quality requirements of animal feedingstuffs (declared content of active substance and feed homogeneity). Material and Methods Two-gram samples were extracted by potassium phosphate buffer solution. Extracts were filtered and directly analysed by HPLC-DAD without further clean-up. Amoxicillin was separated by acetonitrile and 0.01M phosphate buffer (pH 5.0) on a Phenomenex Luna C18 column. Results This method provided average recoveries of 76.1 to 81.6% with coefficients of variation (CV, %) for repeatability and reproducibility in the ranges of 3.7–7.2% and 5.3–7.6%, respectively. The limit of detection was 51.2 mg/kg and limit of quantification was 103.0 mg/kg. Conclusion The method was successfully validated and proved to be efficient, precise, and useful for quantification of amoxicillin in medicated feedingstuffs.

中文翻译:

使用 HPLC-DAD 技术对含药饲料中阿莫西林进行定量的内部验证方法

摘要 简介 开发并验证了一种用于测定加药饲料中阿莫西林的高效液相色谱-二极管阵列检测器 (HPLC-DAD) 方法。该方法用于调查动物饲料的质量要求(活性物质申报含量和饲料均质性)。材料与方法 用磷酸钾缓冲溶液提取两克样品。过滤提取物并通过 HPLC-DAD 直接分析,无需进一步净化。在 Phenomenex Luna C18 柱上用乙腈和 0.01M 磷酸盐缓冲液 (pH 5.0) 分离阿莫西林。结果 该方法的平均回收率为 76.1% 至 81.6%,重复性和再现性的变异系数 (CV, %) 分别在 3.7-7.2% 和 5.3-7.6% 范围内。检出限为 51。2 mg/kg,定量限为 103.0 mg/kg。结论 该方法已成功验证,证明其高效、准确,可用于药用饲料中阿莫西林的定量分析。
更新日期:2020-06-20
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