lncRNA expression profiles related to apoptosis and autophagy in peripheral blood mononuclear cells of patients with rheumatoid arthritis.,FEBS Open Bio

当前位置： X-MOL 学术 › FEBS Open Bio › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

lncRNA expression profiles related to apoptosis and autophagy in peripheral blood mononuclear cells of patients with rheumatoid arthritis.
FEBS Open Bio ( IF 2.8 ) Pub Date : 2020-07-22 , DOI: 10.1002/2211-5463.12913
Jianting Wen ₁ , Jian Liu _{2,

3} , Hui Jiang ₂ , Lei Wan _{2,

3} , Ling Xin _{2,

3} , Yue Sun _{2,

3} , Pingheng Zhang ₄ , Yanqiu Sun ₁ , Ying Zhang ₁ , Xinlei Du ₁ , Xin Wang ₁ , Jie Wang ₁

Affiliation

Long noncoding RNAs (lncRNAs) are >200‐bp molecules that do not generally code for proteins. Human lncRNAs have well‐characterized roles in gene expression regulation, particularly with regard to protein‐coding genes, and their dysregulation has been linked to disease. Here, we set out to investigate changes in the expression of lncRNAs related to apoptosis and autophagy in the peripheral blood mononuclear cells (PBMCs) of rheumatoid arthritis (RA). In addition, we aimed to correlate lncRNA expression profiles with clinical indexes and self‐perception of patients (SPP). To this end, we employed RNA sequencing of lncRNAs in PBMCs from three patients with RA and three healthy controls. We used bioinformatics to screen several dysregulated lncRNAs related to apoptosis and autophagy. To validate key lncRNA candidates, we performed quantitative reverse transcriptase–PCR on 20 patients with RA and 20 healthy controls. We found the expression of seven lncRNAs (MAPKAPK5‐AS1, ENST00000619282, C5orf17, LINC01189, LINC01006, DSCR9 and MIR22HG) was significantly altered in PBMCs of patients with RA. Receiver operating characteristic curve analysis suggested that MIR22HG [area under the curve (AUC) = 0.846, P = 0.000], DSCR9 (AUC = 0.783, P = 0.005), LINC01189 (AUC = 0.677, P = 0.034), MAPKAPK5‐AS1 (AUC = 0.644, P = 0.025) and ENST00000619282 (AUC = 0.636, P = 0.043) are potential biomarkers of RA. Spearman's correlation analysis revealed selected lncRNAs correlated with clinical indexes and SPP. Therefore, we highlight that some lncRNAs related to apoptosis and autophagy may serve as potential biomarkers for diagnosis and monitoring of RA progression, which also correlate with several clinical indexes and SPP.

中文翻译：

类风湿关节炎患者外周血单个核细胞中与凋亡和自噬相关的lncRNA表达谱。

长非编码 RNA (lncRNA) 是 >200 bp 的分子，通常不编码蛋白质。人类lncRNA在基因表达调控中具有明确的作用，特别是在蛋白质编码基因方面，它们的失调与疾病有关。在这里，我们着手研究类风湿性关节炎（RA）外周血单核细胞（PBMC）中与凋亡和自噬相关的lncRNA表达变化。此外，我们的目的是将 lncRNA 表达谱与临床指标和患者自我感知 (SPP) 相关联。为此，我们对三名 RA 患者和三名健康对照者的 PBMC 中的 lncRNA 进行了 RNA 测序。我们利用生物信息学筛选了几种与细胞凋亡和自噬相关的失调lncRNA。为了验证关键的 lncRNA 候选物，我们对 20 名 RA 患者和 20 名健康对照者进行了定量逆转录酶 PCR。我们发现 RA 患者的 PBMC 中 7 个 lncRNA（MAPKAPK5-AS1、ENST00000619282、C5orf17、LINC01189、LINC01006、DSCR9 和 MIR22HG）的表达显着改变。受试者工作特征曲线分析表明，MIR22HG [曲线下面积 (AUC) = 0.846， P = 0.000]、DSCR9 (AUC = 0.783， P = 0.005)、LINC01189 (AUC = 0.677， P = 0.034)、MAPKAPK5-AS1 ( AUC = 0.644， P = 0.025）和 ENST00000619282（AUC = 0.636， P = 0.043）是 RA 的潜在生物标志物。 Spearman 的相关性分析显示，选定的 lncRNA 与临床指标和 SPP 相关。因此，我们强调一些与细胞凋亡和自噬相关的lncRNA可能作为诊断和监测RA进展的潜在生物标志物，这也与一些临床指标和SPP相关。

更新日期：2020-07-22

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11