Optical coherence tomography (OCT) is an optical imaging modality that has been investigated for real-time surgical margin evaluation in human breast cancer patients. Previous veterinary OCT studies have been limited to surgical margin imaging for soft tissue sarcoma (STS) tumours. To the authors knowledge, OCT has never been used to characterize or evaluate other types of neoplasia in dogs. The goal of this study was to characterize the OCT imaging appearance of apocrine gland anal sac adenocarcinoma (AGASACA) in excised ex vivo specimens from five client-owned dogs. All excised tissue surgical margins were imaged using a clinical spectral domain OCT system and two to four areas suspicious for incomplete surgical margins were selected. These areas were inked and sections were trimmed for histopathology. This enabled OCT imaging from each area of interest to be compared with corresponding H&E stained histology imaging from the same location. OCT was able to identify the presence of AGASACA at or within 1 mm of the surgical margin in all areas of interest. AGASACA, similar to the previously described canine STS, generated a dense, highly scattering image without any specific textural architecture. This study was able to validate the ability of OCT to accurately identify another type of tumour presence at or close to the surgical margin in the dog. Further study is needed to assess OCT accuracy at identifying other tumour types in dogs to understand its potential clinical applications.

中文翻译：

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犬大汗腺肛门腺癌切除肿瘤的光学相干断层扫描成像

光学相干断层扫描 (OCT) 是一种光学成像模式，已针对人类乳腺癌患者的实时手术切缘评估进行了研究。以前的兽医 OCT 研究仅限于软组织肉瘤 (STS) 肿瘤的手术边缘成像。据作者所知，OCT 从未用于表征或评估狗的其他类型的瘤形成。本研究的目的是表征离体切除的大汗腺肛门囊腺癌 (AGASACA) 的 OCT 成像外观五只客户拥有的狗的标本。使用临床光谱域 OCT 系统对所有切除的组织手术切缘进行成像，并选择了两到四个可疑的不完整手术切缘区域。这些区域被涂上墨水，切片被修整用于组织病理学。这使得来自每个感兴趣区域的 OCT 成像能够与来自同一位置的相应 H&E 染色组织学成像进行比较。OCT 能够在所有感兴趣区域的手术边缘处或 1 毫米内识别出 AGASACA 的存在。AGASACA 与之前描述的犬类 STS 类似，生成了密集、高度散射的图像，没有任何特定的纹理结构。这项研究能够验证 OCT 准确识别狗手术切缘处或附近存在的另一种类型肿瘤的能力。