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Heptabladed β‐propeller lectins PLL2 and PHL from Photorhabdus spp. recognize O‐methylated sugars and influence the host immune system
The FEBS Journal ( IF 5.5 ) Pub Date : 2020-06-19 , DOI: 10.1111/febs.15457
Eva Fujdiarová 1, 2 , Josef Houser 1, 2 , Pavel Dobeš 2, 3 , Gita Paulíková 1, 2 , Nikolay Kondakov 4 , Leonid Kononov 4 , Pavel Hyršl 3 , Michaela Wimmerová 1, 2, 5
Affiliation  

O‐methylation is an unusual sugar modification with a function that is not fully understood. Given its occurrence and recognition by lectins involved in the immune response, methylated sugars were proposed to represent a conserved pathogen‐associated molecular pattern. We describe the interaction of O‐methylated saccharides with two β‐propeller lectins, the newly described PLL2 from the entomopathogenic bacterium Photorhabdus laumondii, and its homologue PHL from the related human pathogen Photorhabdus asymbiotica. The crystal structures of PLL2 and PHL revealed up to 10 out of 14 potential binding sites per protein subunit to be occupied with O‐methylated structures. The avidity effect strengthens the interaction by 4 orders of magnitude. PLL2 and PHL also interfere with the early immune response by modulating the production of reactive oxygen species and phenoloxidase activity. Since bacteria from Photorhabdus spp. have a complex life cycle involving pathogenicity towards different hosts, the involvement of PLL2 and PHL might contribute to the pathogen overcoming insect and human immune system defences in the early stages of infection.

中文翻译:

来自Photorhabdus spp的Hetabladedβ螺旋桨凝集素PLL2和PHL。识别O-甲基化糖并影响宿主免疫系统

O-甲基化是一种不寻常的糖修饰,其功能尚不完全清楚。鉴于甲基化糖的出现和被参与免疫应答的凝集素识别,有人提出了甲基化糖代表一种与病原体相关的保守分子模式。我们描述了O-甲基化糖与两种β-螺旋桨凝集素的相互作用,新描述的来自致病性细菌劳氏光子菌的PLL2及其来自相关人类病原体不对称光合菌的同系物PHL 。PLL2和PHL的晶体结构揭示了每个蛋白质亚基14个潜在结合位点中最多10个被O占据甲基化结构。亲和力效应将相互作用增强了4个数量级。PLL2和PHL还通过调节活性氧的产生和酚氧化酶的活性来干扰早期的免疫反应。由于细菌来自Photorhabdus spp。由于具有复杂的生命周期,涉及对不同宿主的致病性,因此在感染的早期阶段,PLL2和PHL的参与可能有助于病原体克服昆虫和人类免疫系统的防御。
更新日期:2020-06-19
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