Aqueous RAFT Synthesis of Low Molecular Weight Anionic Polymers for Determination of Structure/Binding Interactions with Gliadin.,Macromolecular Bioscience

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Aqueous RAFT Synthesis of Low Molecular Weight Anionic Polymers for Determination of Structure/Binding Interactions with Gliadin.
Macromolecular Bioscience ( IF 4.4 ) Pub Date : 2020-06-22 , DOI: 10.1002/mabi.202000125
Ashleigh N Bristol ₁ , Brooke P Carpenter ₁ , Ashley N Davis ₁ , Lisa K Kemp ₁ , Vijayaraghavan Rangachari ₂ , Shahid Karim ₃ , Sarah E Morgan ₁

Affiliation

Gliadin, a component of gluten and a known epitope, is implicated in celiac disease (CeD) and results in an inflammatory response in CeD patients when consumed. Acrylamide‐based polyelectrolytes are employed as models to determine the effect of molecular weight and pendent group on non‐covalent interaction modes with gliadin in vitro. Poly(sodium 2‐acrylamido‐2‐methylpropane sulfonate) and poly(sodium 3‐methylpropyl‐3‐butanoate) are synthesized via aqueous reversible addition fragmentation chain transfer (aRAFT) polymerization and characterized by gel permeation chromatography‐multiangle laser light scattering. The polymer/gliadin blends are examined via circular dichroism, zeta potential measurements, 8‐anilinonaphthalene‐1‐sulfonic acid fluorescence spectroscopy, and dynamic light scattering. Acrylamide polymers containing strong anionic pendent groups have a profound effect on gliadin secondary structure and solution behavior below the isoelectric point, while polymers containing hydrophobic character only have a minor impact. The polymers have little effect on gliadin secondary structure and solution behavior at the isoelectric point.

中文翻译：

用于测定与麦醇溶蛋白的结构/结合相互作用的低分子量阴离子聚合物的水性 RAFT 合成。

麦胶蛋白是麸质的一种成分和已知表位，与乳糜泻 (CeD) 相关，食用后会导致 CeD 患者的炎症反应。丙烯酰胺基聚电解质被用作模型，以确定分子量和侧基对体外与麦醇溶蛋白的非共价相互作用模式的影响。聚（2-丙烯酰胺-2-甲基丙烷磺酸钠）和聚（3-甲基丙基-3-丁酸钠）通过水性可逆加成断裂链转移（aRAFT）聚合合成，并通过凝胶渗透色谱-多角激光散射表征。通过圆二色性、zeta 电位测量、8-苯胺萘-1-磺酸荧光光谱和动态光散射检测聚合物/麦醇溶胶混合物。含有强阴离子侧基的丙烯酰胺聚合物对麦醇溶蛋白二级结构和等电点以下的溶液行为具有深远的影响，而含有疏水特性的聚合物仅具有较小的影响。在等电点，聚合物对麦醇溶蛋白的二级结构和溶液行为几乎没有影响。

更新日期：2020-06-22

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