Cell-Laden Hydrogels for Multikingdom 3D Printing.,Macromolecular Bioscience

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Cell-Laden Hydrogels for Multikingdom 3D Printing.
Macromolecular Bioscience ( IF 4.4 ) Pub Date : 2020-06-22 , DOI: 10.1002/mabi.202000121
Trevor G Johnston ₁ , Jacob P Fillman ₁ , Hans Priks ₂ , Tobias Butelmann ₂ , Tarmo Tamm ₂ , Rahul Kumar ₂ , Petri-Jaan Lahtvee ₂ , Alshakim Nelson ₁

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Living materials are created through the embedding of live, whole cells into a matrix that can house and sustain the viability of the encapsulated cells. Through the immobilization of these cells, their bioactivity can be harnessed for applications such as bioreactors for the production of high‐value chemicals. While the interest in living materials is growing, many existing materials lack robust structure and are difficult to pattern. Furthermore, many living materials employ only one type of microorganism, or microbial consortia with little control over the arrangement of the various cell types. In this work, a Pluronic F127‐based hydrogel system is characterized for the encapsulation of algae, yeast, and bacteria to create living materials. This hydrogel system is also demonstrated to be an excellent material for additive manufacturing in the form of direct write 3D‐printing to spatially arrange the cells within a single printed construct. These living materials allow for the development of incredibly complex, immobilized consortia, and the results detailed herein further enhance the understanding of how cells behave within living material matrices. The utilization of these materials allows for interesting applications of multikingdom microbial cultures in immobilized bioreactor or biosensing technologies.

中文翻译：

用于多王国3D打印的载有细胞的水凝胶。

通过将完整的活细胞嵌入到可以容纳和维持被包封细胞的活力的基质中，可以产生活物质。通过固定这些细胞，可以利用它们的生物活性，例如用于生产高价值化学品的生物反应器。尽管人们对生物材料的兴趣不断增长，但许多现有材料却缺乏坚固的结构，并且难以图案化。此外，许多生物仅采用一种微生物或微生物群落，而对各种细胞类型的排列几乎没有控制。在这项工作中，基于Pluronic F127的水凝胶系统的特征是将藻类，酵母和细菌包封起来以产生生物材料。这种水凝胶系统还以直接书写3D打印的形式被证明是一种出色的增材制造材料，可在单个印刷结构中将细胞空间排列。这些生物材料可以发展出难以置信的复杂，固定化的财团，并且本文详述的结果进一步增强了对细胞在生物材料基质中的行为的理解。这些材料的利用允许在固定的生物反应器或生物传感技术中多王国微生物培养物的有趣应用。并且本文详述的结果进一步增强了对细胞在活物质基质中行为的理解。这些材料的利用允许在固定的生物反应器或生物传感技术中多王国微生物培养物的有趣应用。并且本文详述的结果进一步增强了对细胞在活物质基质中行为的理解。这些材料的利用允许在固定的生物反应器或生物传感技术中多王国微生物培养物的有趣应用。

更新日期：2020-06-22

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