The Effect of Polymer Dots During Mammalian Early Embryo Development and Their Biocompatibility on Maternal Health.,Macromolecular Bioscience

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The Effect of Polymer Dots During Mammalian Early Embryo Development and Their Biocompatibility on Maternal Health.
Macromolecular Bioscience ( IF 4.4 ) Pub Date : 2020-06-22 , DOI: 10.1002/mabi.202000128
Juxiang Zhang ₁ , Fei Teng ₁ , Shiyi Tang ₁ , Yufan Zhang ₁ , Yixiao Guo ₁ , Jingru Li ₁ , Yuqiao Li ₁ , Chunfu Zhang ₁ , Liqin Xiong ₁

Affiliation

Conjugated polymer dots have excellent fluorescence properties in terms of their structural diversity and functional design, showing broad application prospects in the fields of biological imaging and biosensing. Polymer dots contain no heavy metals and are thought to be of low toxicity and good biocompatibility. Therefore, systematic studies on their potential toxicity are needed. Herein, the biocompatibility of poly[(9,9‐dioctylfluorenyl‐2,7diyl)‐co‐(1,4‐benzo‐{2,1′,3}‐thiadiazole)],10% benzothiadiazole(y) (PFBT) and poly[2‐methoxy‐5‐(2‐ethylhexyloxy)‐1,4‐phenylenevinylene] (MEH‐PPV) polymer dots on early embryo development as well as maternal health is studied in detail. The results show that prepared polymer dots are dose‐dependently toxic to preimplantation embryos, and low‐dose polymer dots can be used for cell labeling of early embryos without affecting the normal development of embryos into blastocysts. In addition, the in vivo distribution data show that the polymer dots accumulate mainly in the maternal liver, spleen, kidney, placenta, ovary, and lymph nodes of the pregnant mice. Histopathological examination and blood biochemical tests demonstrate that exposure of the maternal body to polymer dots at a dosage of 14 µg g⁻¹ does not affect the normal function of the maternal organs and early fetal development. The research provides a safe basis for the wide application of polymer dots.

中文翻译：

哺乳动物早期胚胎发育过程中的聚合物点及其生物相容性对孕产妇健康的影响。

共轭聚合物点在结构多样性和功能设计方面均具有出色的荧光特性，在生物成像和生物传感领域显示出广阔的应用前景。聚合物点不含重金属，被认为具有低毒性和良好的生物相容性。因此，需要对其潜在毒性进行系统研究。在此，聚的生物相容性[（9,9-二辛基芴2,7diyl） -共-（1,4-苯并-{2,1'，3}-噻二唑）]，10％苯并噻二唑（y）（PFBT）和聚[2-甲氧基-5-（2-乙基己氧基）-1,4-苯撑乙烯撑]（MEH-PPV）聚合物点对早期胚胎发育以及孕妇健康的影响。结果表明，制备的聚合物点对植入前的胚胎具有剂量依赖性的毒性，低剂量的聚合物点可用于早期胚胎的细胞标记，而不会影响胚胎正常发育为胚泡。另外，体内分布数据表明聚合物点主要在怀孕小鼠的母体肝脏，脾脏，肾脏，胎盘，卵巢和淋巴结中积累。组织病理学检查和血液生化检查表明，母体以14 µg g ^-1的剂量暴露于聚合物点不会影响母体器官的正常功能和胎儿的早期发育。该研究为聚合物点的广泛应用提供了安全的基础。

更新日期：2020-06-22

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