BACKGROUND The experiment investigated the chronic effect on the compositions and proportions of cecal microbiota of laying hens for 12 weeks after consuming two genetically modified (GM) corn containing the maroACC gene from Agrobacterium tumefaciens strain (CC) and the mCry1Ac gene from Bacillus thuringiensis strain (BT) in comparison with the isogenic corn (CT). RESULTS A total of 72 hens were randomly assigned to the CT corn-based diet, CC corn-based diet, and BT corn-based diet. The absolute weights of abdominal fat, breast, thigh meat, and organ weight were not affected by the dietary treatment. High-throughput 16S rRNA gene sequencing revealed a few differences in the composition of the cecal microbiota among the treatments. The only difference for bacterial family was that the cecal abundance of Porphyromonadaceae (3.46 versus 2.11%; P = 0.073) tended to be higher for birds consumed the CC diet than those birds consumed the CT diet. Birds fed the BT diet tended to have higher abundance of Barnesiella (0.62 versus 0.13%; P = 0.057) and lower abundance of unclassified Ruminococcaceae (0.64 versus 1.19%; P = 0.097) than those fed the CT diet. As considering beneficial intestinal Barnesiella, it decreases and ultimately clears colonization of vancomycin-resistant Enterococcus. The unclassified Ruminococcaceae was a low-frequency and low-abundance bacterial taxa and was not associated with intestinal pathology. CONCLUSION These results indicate similar modulation of cecal microbiota in laying hens by long-term feeding among the transgenic CC corn, BT corn, and non-transgenic corn and provide scientific data for biosafety evaluation of the transgenic corn. This article is protected by copyright. All rights reserved.

中文翻译：

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摄入含有 maroACC 或 mCry1Ac 基因的转基因玉米对蛋鸡盲肠微生物群的调节

背景 本实验研究了两种含有根癌农杆菌 (CC) maroACC 基因和苏云金芽孢杆菌 (Bacillus thuringiensis) 基因 mCry1Ac 基因的转基因玉米对蛋鸡盲肠微生物群组成和比例的慢性影响，持续 12 周。 BT) 与等基因玉米 (CT) 相比。结果 总共 72 只母鸡被随机分配到 CT 玉米基日粮、CC 玉米基日粮和 BT 玉米基日粮。腹部脂肪、胸部、大腿肉和器官重量的绝对重量不受饮食处理的影响。高通量 16S rRNA 基因测序揭示了治疗之间盲肠微生物群组成的一些差异。细菌家族的唯一区别是卟啉单胞菌科的盲肠丰度（3.46 对 2.11%；P = 0. 073）对于食用 CC 饮食的鸟类而言，往往高于食用 CT 饮食的鸟类。与饲喂 CT 日粮的鸡相比，饲喂 BT 日粮的家禽往往具有更高的巴氏杆菌丰度（0.62 比 0.13%；P = 0.057），而未分类瘤胃球菌科的丰度更低（0.64 比 1.19%；P = 0.097）。考虑到有益的肠道巴氏杆菌，它减少并最终清除耐万古霉素肠球菌的定植。未分类的瘤胃球菌科是一种低频、低丰度的细菌类群，与肠道病理学无关。结论这些结果表明，长期饲喂转基因CC玉米、BT玉米和非转基因玉米对蛋鸡盲肠菌群的调节作用相似，为转基因玉米的生物安全性评价提供了科学依据。本文受版权保护。版权所有。