The ability to precisely measure and monitor temperature at high resolution at the nanoscale is an important task for better understanding the thermodynamic properties of functional entities at the nanoscale in complex systems, or at the level of a single cell. However, the development of high‐resolution and robust thermal nanosensors is challenging. The design, assembly, and characterization of a group of thermal‐responsive deoxyribonucleic acid (DNA) joints, consisting of two interlocked double‐stranded DNA (dsDNA) rings, is described. The DNA nanojoints reversibly switch between the static and mobile state at different temperatures without a special annealing process. The temperature response range of the DNA nanojoint can be easily tuned by changing the length or the sequence of the hybridized region in its structure, and because of its interlocked structure the temperature response range of the DNA nanojoint is largely unaffected by its own concentration; this contrasts with systems that consist of separated components.

中文翻译：

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用于可逆热传感的互锁 DNA 纳米接头。

在纳米尺度上以高分辨率精确测量和监测温度的能力是更好地理解复杂系统中纳米尺度或单细胞水平的功能实体的热力学性质的一项重要任务。然而，高分辨率和稳健的热纳米传感器的开发具有挑战性。描述了一组热响应脱氧核糖核酸 (DNA) 接头的设计、组装和表征，该接头由两个互锁的双链 DNA (dsDNA) 环组成。DNA纳米接头可以在不同温度下在静态和移动状态之间可逆地切换，无需特殊的退火过程。DNA纳米接头的温度响应范围可以通过改变其结构中杂交区域的长度或序列来容易地调节，并且由于其互锁结构，DNA纳米接头的温度响应范围很大程度上不受其自身浓度的影响；这与由独立组件组成的系统形成对比。