The development of novel wastewater platforms should include the analysis of the most critical functional factors including the effects of toxic or inhibitory substances. Due to the novelty of purple phototrophic bacteria (PPB)-based wastewater treatment systems, this analysis has not been done yet in mixed cultures. In this work, various relevant chemical compounds, including aromatic (phenol, 2,4,6-trichlorophenol or 246TCP, 4-nitrophenol or 4CP, sulfathiazole) and aliphatic organics (methanol, trichlorethylene or TCE, oleic acid, ethanol, propionic acid), inorganic salts (ammonium, ClO₃⁻, Na⁺), and metals (Fe³⁺, Fe²⁺, Cu²⁺, Zn²⁺, Ni²⁺, Al³⁺), as well as pH, are analyzed for their effect on mixed PPB cultures in anaerobic photoheterotrophic conditions using acetate as the model organic substrate. The most toxic substances detected were 246TCP, 4NP, Cu²⁺, Fe²⁺ and Ni²⁺, (K_i for activity: 23 ± 2, 97 ± 12, 3.1 ± 0.4, 13 ± 3, 13 ± 1 mg/L, and K_i (or toxicity threshold) for growth: 17 ± 2, (119), 3.5 ± 0.4, (4.8), (22.9) mg/L, respectively). Some substances inhibited the activity more than the growth (sulfathiazole, Ni²⁺ and Fe³⁺), or the growth more than the activity (TCE, 4NP and Fe²⁺). In addition, some organic substrates, such as phenol, ethanol and propionate, specifically inhibited the acetate uptake, being noncompetitive in the case of phenol and ethanol, and most likely competitive in the case of propionate. These findings are relevant for the wastewater treatment and resource recovery applications of the PPB technology, as well as for the upgrading of current models (Photo-Anaerobic Model). In addition, the data will open possibilities to promote the production of specific compounds (as PHA or single-cell proteins) by selectively inhibiting some parts of the PPB metabolism.

新型废水平台的开发应包括对最关键的功能因素的分析，包括有毒或抑制性物质的影响。由于基于紫色光养细菌（PPB）的废水处理系统具有新颖性，因此尚未在混合培养物中进行此分析。在这项工作中，各种相关的化学化合物，包括芳族化合物（苯酚，2,4,6-三氯苯酚或246TCP，4-硝基苯酚或4CP，磺胺噻唑）和脂族有机物（甲醇，三氯乙烯或TCE，油酸，乙醇，丙酸） ，无机盐（铵，CLO ₃ ^- ，钠⁺），和金属（Fe ³⁺，铁²⁺，铜²⁺，锌²⁺，镍²⁺，铝使用乙酸盐作为模型有机底物，在厌氧光异养条件下分析³⁺和pH对混合PPB培养物的影响。所检测到的最剧毒的物质是246TCP，4NP，Cu 2 ⁺，Fe ²⁺和Ni ²⁺（活性的K _i：23± 2、97±12、3.1±0.4、13 ±3、13±1 mg / L ，生长的K _i（或毒性阈值）：分别为17±2，（119），3.5±0.4，（4.8），（22.9）mg / L）。有些物质对活性的抑制作用比其增长（磺胺噻唑，Ni ²⁺和Fe ³⁺）要强，或者对生长的抑制作用比其活性（TCE，4NP和Fe ²⁺）。另外，某些有机底物，例如苯酚，乙醇和丙酸酯，特别抑制了乙酸盐的吸收，在苯酚和乙醇的情况下是无竞争性的，在丙酸酯的情况下极有可能竞争。这些发现与PPB技术的废水处理和资源回收应用以及当前模型（光电厌氧模型）的升级有关。此外，这些数据将通过选择性抑制PPB代谢的某些部分，为促进特定化合物（如PHA或单细胞蛋白）的生产提供可能性。