Senecavirus A (SVA), previously called Seneca Valley virus, belongs to the family Picornaviridae, species Senecavirus A, in the Senecavirus genus, and can cause vesicular lesions in sows and acute death in piglets. In this study, recombinant VP1 and VP2 proteins were expressed in prokaryotic expression system and used to generate eight monoclonal antibodies (mAbs) against VP1 or VP2 protein. And all of the mAbs reacted specifically with SVA virus by both Western blot and indirect immunofluorescence assay (IFA). The resurts showed that all of the epitopes aganist these mAbs were B cell linear epitopes. To map the epitopes, both Western blot and indirect enzyme-linked immunosorbant assay (indirect ELISA) were performed. The epitope ²¹GELAAP²⁶ recognized by mAb 1G9, was likely to be a significant B cell epitope due to the high antigenic index and the fully exposure on the surface of the VP1. Other mAbs were recognized by VP2 protein. MAbs 1E7 and 8E8 recognized the same epitope at ¹²DRVITQT¹⁸, 1A5 recognized the epitope at ⁷¹WTKAVK⁷⁶, 1G2 recognized the epitope at ⁹⁸GGAFTA¹⁰³, 9D2 and 6B11 recognized the same epitope at ¹⁵⁰KSLQELN¹⁵⁶, and 7E4 recognized the epitope at ²⁴⁸YKEGAT²⁵³. Alignment of amino acids revealed that four epitopes were completely conserved among all SVA strains, including ²¹GELAAP²⁶, ⁷¹WTKAVK⁷⁶, ⁹⁸GGAFTA¹⁰³, and ²⁴⁸YKEGAT²⁵³. Interestingly, there were some amino acid mutations in ¹²DRVITQT¹⁸ and ¹⁵⁰KSLQELN¹⁵⁶, but no significant difference was detected on the reaction intensity between epitopes and the corresponding mAbs. This is the first report about the SVA epitopes, which will benefit to the study of viral pathogenic mechanism, vaccine design, as well as the establishment of detection methods.

Senecavirus一（SVA），以前称为塞内卡谷病毒，属于家庭小核糖核酸病毒，种类Senecavirus一个，在Senecavirus属，以及可引起仔猪母猪和急性死亡水泡。在这项研究中，重组VP1和VP2蛋白在原核表达系统中表达，并用于产生八种针对VP1或VP2蛋白的单克隆抗体（mAb）。通过Western印迹和间接免疫荧光测定（IFA），所有mAb都与SVA病毒特异性反应。复活表明，所有这些mAb的抗原决定簇都是B细胞线性抗原决定簇。为了绘制表位，同时进行了蛋白质印迹和间接酶联免疫吸附测定（间接ELISA）。表位²¹由于高抗原指数和VP1表面的完全暴露，被mAb 1G9识别的GELAAP ²⁶可能是重要的B细胞表位。VP2蛋白可识别其他单克隆抗体。单克隆抗体1E7和8E8在¹² DRVITQT ¹⁸处识别相同的表位，1A5在⁷¹ WTKAVK ⁷⁶处识别该表位，1G2在⁹⁸ GGAFTA ¹⁰³处识别该表位，9D2和6B11在¹⁵⁰ KSLQELN ^156处识别该表位，而7E4在²⁴⁸位识别该表位。YKEGAT ²⁵³。氨基酸的比对揭示了4个表位均SVA株中完全保守的，包括²¹ GELAAP ²⁶，⁷¹ WTKAVK ⁷⁶，⁹⁸ GGAFTA ¹⁰³，和²⁴⁸ YKEGAT ²⁵³。有趣的是，在¹² DRVITQT ¹⁸和¹⁵⁰ KSLQELN ^156中存在一些氨基酸突变，但在抗原决定簇和相应mAb之间的反应强度上未发现明显差异。这是关于SVA表位的第一份报告，这将有助于研究病毒的致病机理，疫苗设计以及检测方法的建立。