The presence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in raw milk is a challenge for veterinarians and public health professionals. In this study, we investigated the presence and clonality of S. aureus and MRSA in milk of individual dairy goats with subclinical mastitis reared under the low-input farming system in Greece and determined the isolates’ enterotoxin gene carriage and their ability to form biofilms. S. aureus was isolated from 162 out of the 559 milk samples examined (29 %) and one isolate per S. aureus-positive sample was further characterized. S. aureus isolates were very closely related even among farms of distant geographical regions. Nine S. aureus isolates carried a functional mecA gene and were classified as MRSA. The S. aureus protein A (spa) typing in the MRSA isolates showed that four belonged to spa type t127 (44.4 %), three to t2049 (33.3 %) and two to t7947 (22.2 %). The spa type t7947 is reported for the first time in Greece. The MRSA isolates originated from two very distantly located farms, one located in the island of Skopelos and the other in Central Macedonia. Four of the MRSA isolates carried the staphylococcal enterotoxin genes sea or sec. Most of the isolates (92 % of S. aureus and 77.8 % of the MRSA) possessed moderate or weak biofilm-formation ability. Raw milk from low-input goat herds may serve as a potential vector of antimicrobial-resistant S. aureus to raw-milk consumers.

原料奶中金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌（MRSA）的存在对兽医和公共卫生专业人员构成了挑战。在这项研究中，我们调查了在希腊的低投入耕作制度下饲养的亚临床乳腺炎的个体乳山羊中，牛奶中金黄色葡萄球菌和MRSA的存在和克隆性，并确定了分离株的肠毒素基因运输及其形成生物膜的能力。从所检查的559份牛奶样本中的162份中分离出金黄色葡萄球菌（占29％），每个金黄色葡萄球菌阳性样本中分离出一株。即使在遥远的地理区域的农场之间，金黄色葡萄球菌的分离株也有密切的联系。九金黄色葡萄球菌分离物带有功能性mecA基因，被归类为MRSA。的金黄色葡萄球菌蛋白A（温泉在MRSA）菌株分型表明，4属于温泉类型T127（44.4％），三到t2049（33.3％）和二到t7947（22.2％）。该温泉类型t7947报道在希腊的第一次。MRSA分离株来自两个非常遥远的农场，一个位于斯科派洛斯岛，另一个位于马其顿中部。四个MRSA分离株携带葡萄球菌肠毒素基因sea或sec。多数分离株（92％的金黄色葡萄球菌和77.8％的MRSA）具有中等或较弱的生物膜形成能力。来自低投入山羊群的原料奶可能成为原料奶消费者潜在的抗微生物金黄色葡萄球菌的载体。