The aim of the present study is to evaluate the effect of lithium heparin and K₂EDTA on haematology, biochemical parameters and blood cell morphology of snow trout Schizopyge plagiostomus inhabiting in Kashmir Himalayan region. The haematological parameters such as hemoglobin (Hb), red blood cellcount (RBC), white blood cell (WBC), haematocrit (Hct), erythrocyte indices; mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV), produced certain significant differences in their values with respect to both anticoagulants. Higher values of Hct, MCH and MCV while lower RBC count and MCHC values were recorded with a sample treated with K₂EDTA compared to sample treated with lithium heparin. However, no significant change in Hb and WBC count was observed in the values of samples treated with both the anticoagulants. In the present study, the effect of two anticoagulant in biochemical parameters revealed that the alkaline phosphatase (ALP) and aspartate transaminase (AST) produced significant (P < 0.05) differences. Higher AST and lower ALP activity were recorded in the sample treated with K₂EDTA compared to overall values in serum. Morphometric analysis showed an increase in the RBC perimeter area in samples treated with K₂EDTA as compared to samples treated with lithium heparin. On the basis of above findings, it is recommended that in fish blood analysis heparin as anticoagulant could be useful as compared to K₂ EDTA.

本研究的目的是评估肝素锂和 K ₂ EDTA 对栖息在克什米尔喜马拉雅地区的雪鳟Schizopyge plagiostomus血液学、生化参数和血细胞形态的影响。血液学参数，如血红蛋白（Hb）、红细胞计数（RBC）、白细胞（WBC）、血细胞比容（Hct）、红细胞指数；平均红细胞血红蛋白 (MCH)、平均红细胞血红蛋白浓度 (MCHC)、平均红细胞体积 (MCV) 对两种抗凝剂的值产生了某些显着差异。用 K ₂处理的样品记录的 Hct、MCH 和 MCV 值较高，而 RBC 计数和 MCHC 值较低EDTA 与用肝素锂处理的样品进行比较。然而，在用两种抗凝剂处理的样品的值中没有观察到 Hb 和 WBC 计数的显着变化。在本研究中，两种抗凝剂对生化参数的影响表明碱性磷酸酶 (ALP) 和天冬氨酸转氨酶 (AST) 产生显着差异 (P < 0.05)。与血清中的总体值相比，在用 K ₂ EDTA处理的样品中记录了更高的 AST 和更低的 ALP 活性。形态测定分析显示，与用肝素锂处理的样品相比，用 K ₂ EDTA处理的样品的 RBC 周长面积增加。基于上述发现，建议在鱼血分析中肝素作为抗凝剂与 K 相比可能有用₂乙二胺四乙酸。