Titanium (Ti) and its alloys have been widely adopted as implant materials due to their satisfactory biocompatibility and favorable mechanical property. However, they usually show poor osteogenic ability, hindering its clinical application. Herein, Na₂Ti₃O₇/SrTiO₃ hybrid coatings on Ti were fabricated via one-step alkali etching in a mixed solution of NaOH and Sr(OH)₂ to enhance its osteogenic activity. Scanning electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, and transmission electron microscopy were used to characterize the samples. Biological experiments were conducted to evaluate their osteogenic activity. The results showed Na₂Ti₃O₇ nanowire coatings could be grown on Ti surface and Sr could be incorporated into the coatings. Incorporation of Sr clustered the nanowires and high Sr content lead to the formation of spherical SrTiO₃ with diameter of about 1 μm. Sr²⁺ could continuously release from the samples and the release amount increased with Sr content in the coatings. Although adhesion, spreading, viability and proliferation of osteoblasts on all the samples had no significant difference when compared with Sr-free one, they could significantly promote osteogenic differentiation as verified by enhanced alkaline phosphatase (ALP) activity, collagen secretion, and extracellular matrix mineralization of osteoblasts. Further studies depicted that the expression levels of osteogenic-related genes including ALP, type I collagen and transforming growth factor-β were significantly upregulated in Sr-containing samples. The desirable osteogenic capacity may be ascribed to the synergistic effect of continuous Sr²⁺ release and nanostructured morphology. The samples containing Sr of 5.65–11.07 at.% showed the best osteogenic capacity therefore possess great potential in hard-tissue implant field.

钛（Ti）及其合金由于其令人满意的生物相容性和良好的机械性能而被广泛用作植入材料。但是，它们通常显示出较差的成骨能力，从而阻碍了其临床应用。在本文中，通过在NaOH和Sr（OH）₂的混合溶液中进行一步碱蚀刻来增强Ti上的Na ₂ Ti ₃ O ₇ / SrTiO ₃杂化涂层，以增强其成骨活性。使用扫描电子显微镜，X射线衍射，X射线光电子能谱和透射电子显微镜来表征样品。进行了生物学实验以评估其成骨活性。结果表明Na ₂ Ti ₃O ₇纳米线涂层可以在Ti表面上生长，并且Sr可以掺入涂层中。掺入Sr使纳米线成簇，并且高Sr含量导致形成直径约为1μm的球形SrTiO ₃。Sr ²⁺可以从样品中连续释放，释放量随涂料中Sr含量的增加而增加。尽管与不含Sr的样品相比，所有样品上成骨细胞的粘附力，铺展性，生存力和增殖均无显着差异，但通过增强碱性磷酸酶（ALP）活性，胶原蛋白分泌和细胞外基质矿化作用，它们可以显着促进成骨细胞分化成骨细胞。进一步的研究表明，在含Sr的样品中，成骨相关基因（包括ALP，I型胶原和转化生长因子-β）的表达水平显着上调。理想的成骨能力可归因于连续Sr ²⁺的协同作用释放和纳米结构形态。样品中Sr为5.65–11.07 at。％时显示出最佳的成骨能力，因此在硬组织植入领域具有巨大潜力。