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Defatting of sweet whey by electrodialysis with bipolar membranes: Effect of protein concentration factor
Separation and Purification Technology ( IF 8.6 ) Pub Date : 2020-06-20 , DOI: 10.1016/j.seppur.2020.117248
Mélanie Faucher , Véronique Perreault , Sami Gaaloul , Laurent Bazinet

Every year, sweet whey is generated in huge quantities and its valorization is often accompanied by a preliminary skimming step by centrifugation. However, some residual lipids may still be present after skimming process and cause technological issues. Electrodialysis with bipolar membrane (EDBM) can be used to remove these residual lipids as it simultaneously decreases pH and ionic strength allowing the formation of lipoprotein complexes than can further be recovered. The aim of this work was to test whey protein concentrates (WPCs) with different protein concentration factor (2.27X, 3.34X, 3.56X and 3.80X) during EDBM and to evaluate the impact of the protein concentration factor on the process efficiency, particularly on the defatting rate (when a dilution was applied or not). It appeared that, whatever the protein concentration factor, a dilution was needed to reach higher defatting rates (8.37 ± 1.72% without dilution vs 78.02 ± 0.66% with a 6X dilution for WPC 3.80X), as the EDBM was not sufficient alone to lower ionic strength to promote lipoprotein complex formation. Furthermore, when a dilution was applied after EDBM treatment, a higher protein concentration factor slightly increased the defatting rate (72.76 ± 0.71% for WPC 2.27X vs 78.02 ± 0.66% for WPC 3.80X) as a further lower ionic strength and a higher protein content favorized the lipoprotein complex formation. The increase in the defatting rate was however not proportional to the protein concentration factor. Furthermore, these conditions also allowed proteins to form protein complexes that precipitated along with the lipoprotein complexes. To our best knowledge, it was the first time that protein concentration factor of sweet whey was studied regarding the defatting rate after EDBM.



中文翻译:

双极膜电渗析法去除甜乳清的脱脂:蛋白质浓缩因子的影响

每年,都会大量生产甜乳清,其增值通常伴随着通过离心的初步脱脂步骤。但是,撇脂过程后仍可能存在一些残留的脂质,并引起技术问题。双极膜电渗析(EDBM)可用于去除这些残留的脂质,因为它同时降低了pH和离子强度,从而形成了脂蛋白复合物,而这种复合物可进一步回收。这项工作的目的是在EDBM期间测试具有不同蛋白质浓缩因子(2.27X,3.34X,3.56X和3.80X)的乳清浓缩蛋白(WPC),并评估蛋白质浓缩因子对加工效率的影响,特别是脱脂率(是否使用稀释液)。看来,不管蛋白质浓缩因子是多少,需要稀释才能达到更高的脱脂率(无稀释时为8.37±1.72%,而WPC为3.80X时为6.X稀释时为78.02±0.66%),因为EDBM本身不足以降低离子强度来促进脂蛋白复合物的形成。此外,在EDBM处理后使用稀释液时,较高的蛋白质浓缩系数会略微增加脱脂率(WPC 2.27X为72.76±0.71%,而WPC 3.80X为78.02±0.66%),因为离子强度进一步降低,蛋白质含量更高含量有利于脂蛋白复合物的形成。然而,脱脂率的增加与蛋白质浓缩因子不成比例。此外,这些条件还允许蛋白质形成与脂蛋白复合物一起沉淀的蛋白质复合物。据我们所知,

更新日期:2020-06-30
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