A novel magnetic/plasmonic-assisted fluoro-immunoassay system is developed for the detection of influenza virus using magnetic-derivatized plasmonic molybdenum trioxide quantum dots (MP-MoO₃ QDs) as the plasmonic/magnetic agent and fluorescent graphitic carbon nitride quantum dots (gCNQDs) as the monitoring probe. Specific antibody against influenza A virus was conjugated onto the surface of MP-MoO₃ QDs and gCNQDs, respectively. In the presence of influenza A virus (as the test virus), a core-satellite immunocomplex is formed between the antibody-conjugated nanomaterials (Ab-MP-MoO₃ QDs and Ab-gCNQDs) and their interaction resulted in the modulation and gradual enhancement of the fluorescence intensity of the detection probe with the influenza virus concentration-dependent increase. In addition, PL change without influenza A virus was not observed. Limits of detection of 0.25 and 0.9 pg/mL were achieved for Influenza virus A/New Caledonia (20/99/IVR/116) (H1N1) detection in deionized water and human serum, respectively. Clinically isolated influenza virus A/Yokohama (110/2009) (H3N2) was detected in the range of 45 – 25,000 PFU/mL, with a limit of detection ca 45 PFU/mL (as opposed to a minimum of 5000 PFU/mL for a commercial test kit). This developed biosensor provides a robust, sensitive as well as a selective platform for influenza virus detection.

开发了一种新型磁性/等离子体辅助荧光免疫测定系统，使用磁性衍生等离子体三氧化钼量子点 (MP-MoO ₃ QDs) 作为等离子体/磁性剂和荧光石墨氮化碳量子点 (gCNQDs)检测流感病毒) 作为监测探头。针对甲型流感病毒的特异性抗体分别结合到 MP-MoO ₃ QDs 和 gCNQDs 的表面上。在甲型流感病毒（作为测试病毒）存在的情况下，抗体结合的纳米材料（Ab-MP-MoO ₃QDs和Ab-gCNQDs）及其相互作用导致检测探针的荧光强度随流感病毒浓度依赖性增加而调节和逐渐增强。此外，没有观察到没有甲型流感病毒的PL变化。去离子水和人血清中甲型流感病毒/新喀里多尼亚 (20/99/IVR/116) (H1N1) 的检测限分别为 0.25 和 0.9 pg/mL。临床分离的流感病毒 A/Yokohama (110/2009) (H3N2) 的检测范围为 45 – 25,000 PFU/mL，检出限约为 45 PFU/mL（相比之下，最低检测限为 5000 PFU/mL）商业测试套件）。这种开发的生物传感器为流感病毒检测提供了一个强大、灵敏和选择性的平台。