This report outlines a versatile novel quenching electrochemiluminescence immunosensor based on electrochemiluminescence (ECL) emission with aggregation induced emission (AIE) from TP−COOH NCs, which was utilized by poly-(styrene-co-maleicanhydride) to wrap on tetraphenylethylene nanocrystals. On the one hand, the cathode emission excited at -1.15 V could reduce emission produced by oxygen from high potential stimulation, on the other hand, such low potential can further protect the activity of immune molecules. Meanwhile, the coating can reduce interface impedance and completed functionalization. Herein, based on the synergistic effect of electron transfer and energy transfer between TP−COOH NCs and iron doped hydroxyapatite (Fe-HAP), the high efficiency of this ECL sensing platform can be achieved. The proposed ECL immunosensor for detection of trace Cytokeratin 19 fragment 21−1 (CYFRA21−1), exhibited a satisfied detection limit low to 0.01471 pg/mL (S/N = 3). As a result, this approach not only enriches the ECL strategies study to provide a feasible method for foundational clinical examination but also expands the potential application of ECL sensors in biological testing and clinical high-throughput diagnosis.

本报告概述了一种基于TP-COOH NCs的电致化学发光（ECL）发射和聚集诱导发射（AIE）的多功能新型猝灭电致化学发光免疫传感器，其被聚（苯乙烯-马来酸酐）用于包裹在四苯基乙烯纳米晶体上。一方面，以-1.15 V激发的阴极发射可以减少高电位刺激下氧气产生的发射，另一方面，这种低电位可以进一步保护免疫分子的活性。同时，该涂层可以降低界面阻抗并完成功能化。在此，基于TP-COOH NC和掺杂铁的羟基磷灰石（Fe-HAP）之间的电子转移和能量转移的协同效应，可以实现此ECL传感平台的高效率。S / N  = 3）。结果，这种方法不仅丰富了ECL策略研究，为基础临床检查提供了可行的方法，而且扩大了ECL传感器在生物测试和临床高通量诊断中的潜在应用。