Abstract A facile and efficient electrochemical biosensing platform based on screen printed carbon electrode (SPCE) modified with three-dimensional (3D) nanocomposite consists of reduced graphene oxide (RGO) with the insertion of silver nanowires (AgNWs) followed by the anchoring of silver nanoparticles (AgNPs) is constructed as RGO/AgNWs/AgNPs/SPCE for the simultaneous determination of dopamine (DA), uric acid (UA) and estriol (EST). The morphology characteristic and surface elemental composition of RGO/AgNWs/AgNPs nanocomposite are investigated by field-emission scanning electron microscope, transmission electron microscope and X-ray photoelectron spectroscope. Cyclic voltammetry, electrochemical impedance spectroscopy, linear sweep voltammetry and differential pulse voltammetry are utilized to explore the electrochemical performances of the constructed electrodes. Due to abundant active sites and excellent electrocatalytic activity of the nanocomposite, the RGO/AgNWs/AgNPs/SPCE sensor exhibits well-resolved oxidation peaks and enhanced oxidation peak currents in the ternary mixture of DA, UA and EST with respective linear response ranges of 0.6 to 50 μM, 1 to 100 μM and 1 to 90 μM and detection limits (S/N = 3) of 0.16 μM, 0.58 μM and 0.58 μM, respectively. Moreover, the constructed biosensor exhibits good selectivity, reproducibility and stability, and excellent performance in determining DA, UA and EST in synthetic urine samples with excellent recovery. The results reveal that the RGO/AgNWs/AgNPs nanocomposite is a promising candidate for advanced electrode material in electrochemical sensing field and possesses great application prospects in further sensing researches.

中文翻译：

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基于还原氧化石墨烯、银纳米线和银纳米颗粒在三元 3D 纳米复合材料中的协同作用，同时测定母体尿液样品中的多巴胺、尿酸和雌三醇

摘要 基于丝网印刷碳电极 (SPCE) 的简易高效电化学生物传感平台由三维 (3D) 纳米复合材料改性，由还原氧化石墨烯 (RGO) 和银纳米线 (AgNW) 的插入以及银纳米颗粒的锚定组成。 (AgNPs) 被构建为 RGO/AgNWs/AgNPs/SPCE，用于同时测定多巴胺 (DA)、尿酸 (UA) 和雌三醇 (EST)。通过场发射扫描电子显微镜、透射电子显微镜和X射线光电子能谱仪研究了RGO/AgNWs/AgNPs纳米复合材料的形貌特征和表面元素组成。循环伏安法、电化学阻抗谱、利用线性扫描伏安法和差分脉冲伏安法来探索构建电极的电化学性能。由于纳米复合材料丰富的活性位点和优异的电催化活性，RGO/AgNWs/AgNPs/SPCE 传感器在 DA、UA 和 EST 的三元混合物中表现出良好分辨的氧化峰和增强的氧化峰电流，各自的线性响应范围为 0.6至 50 μM、1 至 100 μM 和 1 至 90 μM，检测限 (S/N = 3) 分别为 0.16 μM、0.58 μM 和 0.58 μM。此外，构建的生物传感器具有良好的选择性、重现性和稳定性，在测定合成尿样中的 DA、UA 和 EST 方面表现出色，回收率高。